Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide

	Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide
	Fu, Xiuli 1,2; Chen, Lingxin1 ; Li, Jinhua1
发表期刊	ANALYST
ISSN	0003-2654
	2012
卷号	137 期号:16 页码:3653-3658
关键词	Sensing Strategy Quantification Chromatography Sensitivity Surface Sensor Probe Assay Hg2++
产权排序	[Fu, Xiuli; Chen, Lingxin; Li, Jinhua] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Fu, Xiuli] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
通讯作者	Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China.,[email protected]
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	A novel colorimetric method was developed for ultrasensitive detection of heparin based on self-assembly of gold nanoparticles (AuNPs) onto the surface of graphene oxide (GO). Polycationic protamine was used as a medium for inducing the self-assembly of citrate-capped AuNPs on GO through electrostatic interaction, resulting in a shift in the surface plasmon resonance (SPR) absorption of AuNPs and exhibiting a blue color. Addition of polyanionic heparin disturbed the self-assemble of AuNPs due to its strong affinity to protamine. With the increase of heparin concentration, the amounts of self-assembly AuNPs decreased and the color changed from blue to red in solution. Therefore, a "blue-to-red" colorimetric sensing strategy based on self-assembly of AuNPs could be established for heparin detection. Compared with the commonly reported aggregation-based methods ("red-to-blue"), the color change from blue to red was more eye-sensitive, especially in low concentration of target. Moreover, stronger interaction between protamine and heparin led to distinguish heparin from its analogues as well as various potentially coexistent physiological species. The strategy was simply achieved by the self-assembly nature of AuNPs and the application of two types of polyionic media, showing it to be label-free, simple, rapid and visual. This method could selectively detect heparin with a detection limit of 3.0 ng mL(-1) in standard aqueous solution and good linearity was obtained over the range 0.06-0.36 mu g mL(-1) (R = 0.9936). It was successfully applied to determination of heparin in fetal bovine serum samples as low as 1.7 ng mL(-1) with a linear range of 0-0.8 mu g mL(-1).; A novel colorimetric method was developed for ultrasensitive detection of heparin based on self-assembly of gold nanoparticles (AuNPs) onto the surface of graphene oxide (GO). Polycationic protamine was used as a medium for inducing the self-assembly of citrate-capped AuNPs on GO through electrostatic interaction, resulting in a shift in the surface plasmon resonance (SPR) absorption of AuNPs and exhibiting a blue color. Addition of polyanionic heparin disturbed the self-assemble of AuNPs due to its strong affinity to protamine. With the increase of heparin concentration, the amounts of self-assembly AuNPs decreased and the color changed from blue to red in solution. Therefore, a "blue-to-red" colorimetric sensing strategy based on self-assembly of AuNPs could be established for heparin detection. Compared with the commonly reported aggregation-based methods ("red-to-blue"), the color change from blue to red was more eye-sensitive, especially in low concentration of target. Moreover, stronger interaction between protamine and heparin led to distinguish heparin from its analogues as well as various potentially coexistent physiological species. The strategy was simply achieved by the self-assembly nature of AuNPs and the application of two types of polyionic media, showing it to be label-free, simple, rapid and visual. This method could selectively detect heparin with a detection limit of 3.0 ng mL(-1) in standard aqueous solution and good linearity was obtained over the range 0.06-0.36 mu g mL(-1) (R = 0.9936). It was successfully applied to determination of heparin in fetal bovine serum samples as low as 1.7 ng mL(-1) with a linear range of 0-0.8 mu g mL(-1).
文章类型	Article
资助机构	National Natural Science Foundation of China[20975089, 21105117]; Chinese Academy of Sciences[KZCX2-EW-206]; 100 Talents Program of the Chinese Academy of Sciences
收录类别	SCI
语种	英语
关键词[WOS]	SENSING STRATEGY ; QUANTIFICATION ; CHROMATOGRAPHY ; SENSITIVITY ; SURFACE ; SENSOR ; PROBE ; ASSAY ; HG2+
研究领域[WOS]	Chemistry
WOS记录号	WOS:000306483000009
引用统计	被引频次：46[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/6076
专题	中国科学院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Fu, Xiuli,Chen, Lingxin,Li, Jinhua. Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide[J]. ANALYST,2012,137(16):3653-3658.
APA	Fu, Xiuli,Chen, Lingxin,&Li, Jinhua.(2012).Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide.ANALYST,137(16),3653-3658.
MLA	Fu, Xiuli,et al."Ultrasensitive colorimetric detection of heparin based on self-assembly of gold nanoparticles on graphene oxide".ANALYST 137.16(2012):3653-3658.

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