A pair of degenerate primers was designed to amplify specific DNA fragment of FeSOD using cDNA of Haematococcus pluvialis from the homologous sequences.The middle interesting cDNA fragment was obtained by RT-PCR.The full length of HPFeSOD cDNA was obtained by 5′RACE and 3′RACE.The clone contains 1138 bp nucleotides with an open reading frame(ORF) of 684 bp comprising 227 amino acid residues.The sequence shares a high homologue with the following FeSOD: Dunaliella salina,89%,Chlamydomonas reinhardtii,83%,Volvox carteri f.nagariensis,78%,Ulva fasciata,70%,and Cyanobium sp.PCC 7001,63%.Phylogenetic analysis shows that the FeSOD from H.pluvialis and D.salina are clustered together with genes between fungi and higherplants.The FeSOD of eukaryotic microalgae and higher plants may arise through gene duplication events independently from acommon ancestor.
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