| Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial | |
He, Weiyu1; Feng, Feifei1; Huang, Yong4; Guo, Huanhuan1; Zhang, Songyan2; Li, Zheng2; Liu, Jingze1; Wang, Yipeng3 ; Yu, Haining1,2
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| 发表期刊 | BIOCHIMIE
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| ISSN | 0300-9084 |
| 2012-03-01 | |
| 卷号 | 94期号:3页码:649-655 |
| 关键词 | Amphibian Diversity Anti-microbial Peptide (Amp) Antioxidant Odorrana Tiannanensis |
| 产权排序 | [He, Weiyu; Feng, Feifei; Guo, Huanhuan; Liu, Jingze; Yu, Haining] Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China; [Zhang, Songyan; Li, Zheng; Yu, Haining] Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China; [Wang, Yipeng] Chinese Acad Sci, Biol Resources Lab, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Huang, Yong] Guangxi Bot Garden Med Plants, Nanning 530023, Peoples R China |
| 通讯作者 | Yu, HN (reprint author), Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China. [email protected] ; [email protected] |
| 作者部门 | 生物资源实验室 |
| 英文摘要 | Genus Odorrana, among all amphibians studied, is generally reported to have the most abundant and diversified anti-microbial peptides even from a single individual frog. In our previous work, 46 cDNA sequences encoding precursors of 22 different anti-microbial peptides (AMPs) were characterized from the skin of frog, Odorrana tiannanensis. In this work, we reported the purification of three AMPs from skin secretions of O. tiannanensis. Their amino acid sequences matched well with the sequences deduced from cDNAs and they were designated as Odorranain-C7HSa, Brevinin-1-OT2 and Odorranain-G-OT, respectively. Furthermore, we selected to analyze the four most structurally diversified sequences among the 22 AMPs that are significantly different from all reported AMPs. By structural characterization, three of them were designated as pleurain-E-OT, odorranain-G-OT, odorranain-A-OT, belonging to AMP families already identified. The forth one with a unique 14-mer sequence of AILTTLANWARKFLa and C-terminal amidation represents the prototypes of a new class of amphibian AMP, and thereby named tiannanensin. Such broad diversity in sequences and structures are consistent with other species in Genus Odorrana. Multi-functions of the synthesized four special AMPs were screened, including anti-microbial, antioxidant, cytotoxic and hemolytic activities. The results suggest that these AMPs may employ sophisticated mechanisms of action in host defense in addition to anti-microbial, although their precise contribution to host defense still seems unclear.; Genus Odorrana, among all amphibians studied, is generally reported to have the most abundant and diversified anti-microbial peptides even from a single individual frog. In our previous work, 46 cDNA sequences encoding precursors of 22 different anti-microbial peptides (AMPs) were characterized from the skin of frog, Odorrana tiannanensis. In this work, we reported the purification of three AMPs from skin secretions of O. tiannanensis. Their amino acid sequences matched well with the sequences deduced from cDNAs and they were designated as Odorranain-C7HSa, Brevinin-1-OT2 and Odorranain-G-OT, respectively. Furthermore, we selected to analyze the four most structurally diversified sequences among the 22 AMPs that are significantly different from all reported AMPs. By structural characterization, three of them were designated as pleurain-E-OT, odorranain-G-OT, odorranain-A-OT, belonging to AMP families already identified. The forth one with a unique 14-mer sequence of AILTTLANWARKFLa and C-terminal amidation represents the prototypes of a new class of amphibian AMP, and thereby named tiannanensin. Such broad diversity in sequences and structures are consistent with other species in Genus Odorrana. Multi-functions of the synthesized four special AMPs were screened, including anti-microbial, antioxidant, cytotoxic and hemolytic activities. The results suggest that these AMPs may employ sophisticated mechanisms of action in host defense in addition to anti-microbial, although their precise contribution to host defense still seems unclear. (C) 2011 Elsevier Masson SAS. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Chinese National Natural Science Foundation[30900240, 41076098] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | AMPHIBIAN SKIN ; PEPTIDOMICS ; INDUCTION ; ROLES |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| WOS记录号 | WOS:000301332200008 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5561 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050016, Peoples R China 2.Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Biol Resources Lab, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China 4.Guangxi Bot Garden Med Plants, Nanning 530023, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Weiyu,Feng, Feifei,Huang, Yong,et al. Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial[J]. BIOCHIMIE,2012,94(3):649-655. |
| APA | He, Weiyu.,Feng, Feifei.,Huang, Yong.,Guo, Huanhuan.,Zhang, Songyan.,...&Yu, Haining.(2012).Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial.BIOCHIMIE,94(3),649-655. |
| MLA | He, Weiyu,et al."Host defense peptides in skin secretions of Odorrana tiannanensis: Proof for other survival strategy of the frog than merely anti-microbial".BIOCHIMIE 94.3(2012):649-655. |
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