| Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge | |
Zhang, Linbao1,2; Liu, Xiaoli1,2; Chen, Leilei3; You, Liping1,2; Pei, Dong4; Cong, Ming1 ; Zhao, Jianmin1; Li, Chenghua1; Liu, Dongyan1; Yu, Junbao1; Wu, Huifeng1
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| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 2011-12-01 | |
| 卷号 | 31期号:6页码:831-837 |
| 关键词 | Venerupis Philippinarum Selenium-dependent Glutathione Peroxidase Bacterial Challenge Heavy Metals Benzo[a]Pyrene |
| 产权排序 | [Zhang, Linbao; Liu, Xiaoli; You, Liping; Cong, Ming; Zhao, Jianmin; Li, Chenghua; Liu, Dongyan; Yu, Junbao; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Shandong Prov Key Lab Coastal Zone Environm P, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Linbao; Liu, Xiaoli; You, Liping] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China; [Chen, Leilei] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Peoples R China; [Pei, Dong] China Agr Univ Yantai, Yantai 264670, Peoples R China |
| 通讯作者 | Zhao, JM (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Shandong Prov Key Lab Coastal Zone Environm P, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China |
| 作者部门 | 污染过程与控制实验室 |
| 英文摘要 | Glutathione peroxidases (GPx) are key enzymes in the antioxidant systems of living organisms by catalyzing the reduction of peroxides to non-reactive products. In the present study, the full-length cDNA encoding a selenium-dependent GPx was identified from Venerupis philippinarum (designated as VpSe-GPx), and the spatial and temporal expression patterns post-Vibrio anguillarum, heavy metals and benzo[a]pyrene (B[a]P) challenge were also investigated. VpSe-GPx possessed all the conserved features critical for the fundamental structure and function of glutathione peroxidase. The VpSe-GPx mRNA was found to be most abundantly expressed in hepatopancreas. Vibrio challenge could significantly up-regulate the mRNA expression of VpSe-GPx, and the highest expression level was detected at 24 h post-infection with 6.5-fold increase compared with that in the control group. For heavy metals exposure, the expression of VpSe-GPx was significantly induced by 20, 40 mu g L(-1) Cd and 10, 20 mu g L(-1) Cu but depressed by 10 mu g L(-1) Cd and 40 mu g L(-1) Cu. With regards to B[a]P exposure, the expression of VpSe-GPx mRNA was significantly induced at 48 and 96 h post challenge. All these results suggested that VpSe-GPx was potentially involved in mediating the immune response and antioxidant defense in V. Philippinarum. (C) 2011 Elsevier Ltd. All rights reserved.; Glutathione peroxidases (GPx) are key enzymes in the antioxidant systems of living organisms by catalyzing the reduction of peroxides to non-reactive products. In the present study, the full-length cDNA encoding a selenium-dependent GPx was identified from Venerupis philippinarum (designated as VpSe-GPx), and the spatial and temporal expression patterns post-Vibrio anguillarum, heavy metals and benzo[a]pyrene (B[a]P) challenge were also investigated. VpSe-GPx possessed all the conserved features critical for the fundamental structure and function of glutathione peroxidase. The VpSe-GPx mRNA was found to be most abundantly expressed in hepatopancreas. Vibrio challenge could significantly up-regulate the mRNA expression of VpSe-GPx, and the highest expression level was detected at 24 h post-infection with 6.5-fold increase compared with that in the control group. For heavy metals exposure, the expression of VpSe-GPx was significantly induced by 20, 40 mu g L(-1) Cd and 10, 20 mu g L(-1) Cu but depressed by 10 mu g L(-1) Cd and 40 mu g L(-1) Cu. With regards to B[a]P exposure, the expression of VpSe-GPx mRNA was significantly induced at 48 and 96 h post challenge. All these results suggested that VpSe-GPx was potentially involved in mediating the immune response and antioxidant defense in V. Philippinarum. (C) 2011 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 资助机构 | Chinese Academy of Sciences, Technology Development Program Projects of Shandong Province[2008GG20005006, 2008GG3NS0700]; SDSFC[ZR2009CZ008]; CAS/SAFEA |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CLAM RUDITAPES-DECUSSATUS ; OXIDATIVE STRESS ; CRASSOSTREA-GIGAS ; REACTIVE OXYGEN ; FREE-RADICALS ; DNA-DAMAGE ; EXPRESSION ; IDENTIFICATION ; ANTIOXIDANTS ; INFECTION |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000298569700014 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/5396 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 中国科学院海岸带环境过程与生态修复重点实验室_滨海湿地实验室 中国科学院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Shandong Prov Key Lab Coastal Zone Environm P, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo 315211, Peoples R China 4.China Agr Univ Yantai, Yantai 264670, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Linbao,Liu, Xiaoli,Chen, Leilei,et al. Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2011,31(6):831-837. |
| APA | Zhang, Linbao.,Liu, Xiaoli.,Chen, Leilei.,You, Liping.,Pei, Dong.,...&Wu, Huifeng.(2011).Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge.FISH & SHELLFISH IMMUNOLOGY,31(6),831-837. |
| MLA | Zhang, Linbao,et al."Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge".FISH & SHELLFISH IMMUNOLOGY 31.6(2011):831-837. |
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