| Method of mRNA display for selecting peptides that bind with target enzyme RNA | |
| Yan,Song ; Wang,Zheng ; Yang,Shao-Li ; Zhang,Yi ; Lin,Xiu-Kun | |
| 通讯作者 | Yan, S. ([email protected]) |
| 会议主办者 | IEEE Engineering in Medicine and Biology Society; Wuhan University; Fuzhou University; Nankai University; Overs. Chin. Sch. Environ. Prot. Assoc. (OCSEPA) - |
| 2011 | |
| 会议名称 | 5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011 |
| 会议日期 | 2011-05-13 |
| 会议地点 | Wuhan, China |
| 产权排序 | (1)Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China; (2)School of Environmental Science and Engineering, Dalian Jiaotong University, Dalian 116028, China; (3)Yantai Institute of Coastal Zone Research for Sustainable Development, Chinese Academy of Sciences, Yantai, 264003, China |
| 关键词 | Peptides Bioinformatics Medicine Molecular Biology Rna |
| 摘要 | In vitro display techniques are useful tools for selecting novel ligands that bind to target biomolecules. In this paper, mRNA display method was used to select peptides that bind thymidylate synthase RNA with high affinity from a peptide library. We provide a detailed analysis of the critical steps of an mRNA-display-based selection, including the generation of an mRNA-displayed library, formation of RNA-peptide fusions, and selection of RNA affinity peptides. After optimization of the experimental steps, the efficiency of RNA-linker purification and the reverse transcription was improved, and the unspecified binding peptides were removed. Our study could facilitate the use of mRNA display in the field of biotechnology, medicine and proteomics. © 2011 IEEE. |
| 作者部门 | 生物资源实验室 |
| URL | 查看原文 |
| 收录类别 | EI |
| 文献类型 | 会议论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/4934 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 推荐引用方式 GB/T 7714 | Yan,Song,Wang,Zheng,Yang,Shao-Li,et al. Method of mRNA display for selecting peptides that bind with target enzyme RNA[C],2011. |
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