A simplified, sequential, phosphorus fractionation method

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	A simplified, sequential, phosphorus fractionation method
	GUPPY C. N. MENZIES N. W. MOODY P. W. COMPTON B. L. BLAMEY F. P. C.
发表期刊	Taylor & Francis
	2000
卷号	31 期号:11-14 页码:1981-1991
关键词	Simplification Measurement Method Fractionation Sequential Analysis Extraction Process Soils Economic Aspect Colorimetry Phosphorus Sodium Chlorides Inorganic Element Dyes
英文摘要	Hedley et al. (1982) developed what has become the most widely used (and modified), phosphorus (P) fractionation technique. It consists of sequential extraction of increasingly less phytoavailable P pools. Extracts are centrifuged at up to 25000 g (RCF) and filtered to 0.45 μm to ensure that soil is not lost between extractions. In attempting to transfer this method to laboratories with limited facilities, it was considered that access to high-speed centrifuges, and the cost of frequent filtration may prevent adoption of this P fractionation technique. The modified method presented here was developed to simplify methodology, reduce cost, and therefore increase accessibility of P fractionation technology. It provides quantitative recovery of soil between extractions, using low speed centrifugation without filtration. This is achieved by increasing the ionic strength of dilute extracts, through the addition of NaCI, to flocculate clay particles. Addition of NaCI does not change the amount of P extracted. Flocculation with low speed centrifugation produced extracts comparable with those having undergone filtration (0.025 μm). A malachite green colorimetric method was adopted for inorganic P determination, as this simple manual method provides high sensitivity with negligible interference from other anions. This approach can also be used for total P following digestion, alternatively non-discriminatory methods, such as inductively coupled plasma atomic emission spectroscopy, may be employed.
语种	英语
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/4451
专题	科研共享资源
推荐引用方式 GB/T 7714	GUPPY C. N. MENZIES N. W. MOODY P. W. COMPTON B. L. BLAMEY F. P. C.. A simplified, sequential, phosphorus fractionation method[J]. Taylor & Francis,2000,31(11-14):1981-1991.
APA	GUPPY C. N. MENZIES N. W. MOODY P. W. COMPTON B. L. BLAMEY F. P. C..(2000).A simplified, sequential, phosphorus fractionation method.Taylor & Francis,31(11-14),1981-1991.
MLA	GUPPY C. N. MENZIES N. W. MOODY P. W. COMPTON B. L. BLAMEY F. P. C.."A simplified, sequential, phosphorus fractionation method".Taylor & Francis 31.11-14(2000):1981-1991.

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