| Molecular Characterization of a Novel Big Defensin from Clam Venerupis philippinarum | |
Zhao, Jianmin1 ; Li, Chenghua1,2; Chen, Aiqin1; Li, Lingyun3; Su, Xiurong2; Li, Taiwu2
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| 发表期刊 | PLOS ONE
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| ISSN | 1932-6203 |
| 2010-10-20 | |
| 卷号 | 5期号:10页码:e13480- |
| 关键词 | Oyster Crassostrea-gigas Antimicrobial Peptides Expression Gene Shrimp Family Penaeidins Proteins Mussels Crab |
| 产权排序 | [Zhao, Jianmin; Li, Chenghua; Chen, Aiqin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Li, Chenghua; Su, Xiurong; Li, Taiwu] Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo, Peoples R China; [Li, Lingyun] NE Agr Univ, Coll Anim Sci & Technol, Harbin, Peoples R China |
| 通讯作者 | Zhao, JM, Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China |
| 作者部门 | 污染过程与控制实验室 |
| 英文摘要 | Antimicrobial peptides (AMPs) are important mediators of the primary defense mechanism against microbial invasion. In the present study, a big defensin was identified from Venerupis philippinarum haemocytes (denoted as VpBD) by RACE and EST approaches. The VpBD cDNA contained an open reading frame (ORF) of 285 bp encoding a polypeptide of 94 amino acids. The deduce amino acid sequence of VpBD shared the common features of big defensin including disulfide array organization and helix structure, indicating that VpBD should be a new member of the big defensin family. The mRNA transcript of VpBD was up-regulated significantly during the first 24 hr after Vibrio anguillarum challenge, which was 7.4-fold increase compared to that of the control group. Then the expression decreased gradually from 24 hr to 96 hr, and the lowest expression level was detected at 96 hr post-infection, which was still 3.9-fold higher than that of control. The mature peptide of VpBD was recombined in Escherichia coli and purified for minimum inhibitory concentration (MIC) determination. The rVpBD displayed broad-spectrum inhibitory activity towards all tested bacteria with the highest activity against Staphyloccocus aureus and Pseudomonas putida. These results indicated that VpBD was involved in the host immune response against bacterial infection and might contribute to the clearance of invading bacteria.; Antimicrobial peptides (AMPs) are important mediators of the primary defense mechanism against microbial invasion. In the present study, a big defensin was identified from Venerupis philippinarum haemocytes (denoted as VpBD) by RACE and EST approaches. The VpBD cDNA contained an open reading frame (ORF) of 285 bp encoding a polypeptide of 94 amino acids. The deduce amino acid sequence of VpBD shared the common features of big defensin including disulfide array organization and helix structure, indicating that VpBD should be a new member of the big defensin family. The mRNA transcript of VpBD was up-regulated significantly during the first 24 hr after Vibrio anguillarum challenge, which was 7.4-fold increase compared to that of the control group. Then the expression decreased gradually from 24 hr to 96 hr, and the lowest expression level was detected at 96 hr post-infection, which was still 3.9-fold higher than that of control. The mature peptide of VpBD was recombined in Escherichia coli and purified for minimum inhibitory concentration (MIC) determination. The rVpBD displayed broad-spectrum inhibitory activity towards all tested bacteria with the highest activity against Staphyloccocus aureus and Pseudomonas putida. These results indicated that VpBD was involved in the host immune response against bacterial infection and might contribute to the clearance of invading bacteria. |
| 文章类型 | Article |
| 资助机构 | Chinese Academy of Sciences [kzcx2-yw-225]; NSFC [30901115] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | OYSTER CRASSOSTREA-GIGAS ; ANTIMICROBIAL PEPTIDES ; EXPRESSION ; GENE ; SHRIMP ; FAMILY ; PENAEIDINS ; PROTEINS ; MUSSELS ; CRAB |
| 研究领域[WOS] | Science & Technology - Other Topics |
| WOS记录号 | WOS:000283216400009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/3795 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 2.Ningbo Univ, Fac Life Sci & Biotechnol, Ningbo, Peoples R China 3.NE Agr Univ, Coll Anim Sci & Technol, Harbin, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Jianmin,Li, Chenghua,Chen, Aiqin,et al. Molecular Characterization of a Novel Big Defensin from Clam Venerupis philippinarum[J]. PLOS ONE,2010,5(10):e13480-. |
| APA | Zhao, Jianmin,Li, Chenghua,Chen, Aiqin,Li, Lingyun,Su, Xiurong,&Li, Taiwu.(2010).Molecular Characterization of a Novel Big Defensin from Clam Venerupis philippinarum.PLOS ONE,5(10),e13480-. |
| MLA | Zhao, Jianmin,et al."Molecular Characterization of a Novel Big Defensin from Clam Venerupis philippinarum".PLOS ONE 5.10(2010):e13480-. |
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