Phylogenetic distribution and characterization of conserved C-di-GMP metabolizing proteins in filamentous cyanobacterium Arthrospira

doi:10.1016/j.gene.2024.148643

	Phylogenetic distribution and characterization of conserved C-di-GMP metabolizing proteins in filamentous cyanobacterium Arthrospira
	Wang, Kang 1,2; Li, Wenjun1 ; Cui, Hongli1 ; Qin, Song1
发表期刊	GENE
ISSN	0378-1119
	2024-11-15
卷号	927 页码:11
关键词	Arthrospira c-di-GMP Diguanylate cyclase Phosphodiesterase Extracellular polymeric substances
DOI	10.1016/j.gene.2024.148643
通讯作者	Wang, Kang([email protected]) ; Qin, Song([email protected])
英文摘要	Cyclic diguanosine monophosphate (c-di-GMP) is a second messenger in bacteria that regulates multiple biological functions, including biofilm formation, virulence, and intercellular communication. However, c-di-GMP signaling is virtually unknown in economically important filamentous cyanobacteria, Arthrospira. In this study, we predicted 31 genes encoding GGDEF-domain proteins from A. platensis NIES39 as potential diguanylate cyclases (DGCs). Phylogenetic distribution analysis showed five genes (RS09460, RS04865, RS26155, M01840, and E02220) with highly conserved distribution across 25 Arthrospira strains. Adc1 encoded by RS09460 was further characterized as a typical DGC. By establishing the genetic transformation system of Arthrospira, we demonstrated that the overexpression of Adc1 promoted the production of extracellular polymeric substances (EPS), which in turn caused the aggregation of filaments. We also confirmed that RS04865 and RS26155 may encode active DGCs, while enzymatic activity assays showed that proteins encoded by M01840 and E02220 have phosphodiesterase (PDE) activity. Meta-analysis revealed that the expression profiles of RS09460 and RS04865 were unaffected under 31 conditions, suggesting that they may function as conserved genes in maintaining the basal level of c-di-GMP in Arthrospira. In summary, this report will provide the basis for further studies of c-diGMP signal in Arthrospira.
资助机构	Natural Science Foundation of Shandong Province ; Major Science and Technology Innovation Project of Shandong Province ; High-tech industrialization special project in cooperation between Jilin Province ; Chinese Academy of Sciences ; National Natural Science Foundation of China
收录类别	SCI
语种	英语
关键词[WOS]	VIBRIO-CHOLERAE ; TRANSFORMATION
研究领域[WOS]	Genetics & Heredity
WOS记录号	WOS:001345612500001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/37839
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Wang, Kang; Qin, Song
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Kang,Li, Wenjun,Cui, Hongli,et al. Phylogenetic distribution and characterization of conserved C-di-GMP metabolizing proteins in filamentous cyanobacterium Arthrospira[J]. GENE,2024,927:11.
APA	Wang, Kang,Li, Wenjun,Cui, Hongli,&Qin, Song.(2024).Phylogenetic distribution and characterization of conserved C-di-GMP metabolizing proteins in filamentous cyanobacterium Arthrospira.GENE,927,11.
MLA	Wang, Kang,et al."Phylogenetic distribution and characterization of conserved C-di-GMP metabolizing proteins in filamentous cyanobacterium Arthrospira".GENE 927(2024):11.