Mutations in Growth-Related Genes Induced by EMS Treatment in Scallops

doi:10.3389/fgene.2022.879844

	Mutations in Growth-Related Genes Induced by EMS Treatment in Scallops
	Wang, Caihui 1; Liu, Bo 1; Chen, Min2 ; Ning, Junhao 2; Lu, Xia2 ; Wang, Chunde 1,2
发表期刊	FRONTIERS IN GENETICS
	2022-04-26
卷号	13 页码:13
关键词	EMS mutagen scallop genome sequencing SNP INDEL gene
DOI	10.3389/fgene.2022.879844
通讯作者	Wang, Chunde([email protected])
英文摘要	Background: The goal of genetic breeding is to select variants with mutations that are related to expected traits, such as fast growth. Artificial induction has been widely used to obtain strains with more mutations for further selection. Ethylmethylsulfone (EMS) is one of the most commonly used chemical mutagens in plant and microorganism breeding. However, the application of EMS mutagenesis in shellfish has not been reported. The aim of this study is to evaluate the potential use of EMS as a mutagen in scallop breeding, especially in characterization of mutations in growth-related genes.Results: Our results indicated that hatching of about 50% of fertilized eggs was blocked by treatment with 20 mM EMS for 3 h and the resulted larvae developed normally into adult stages. We then evaluated the mutagenic effects of EMS by sequencing the genomes of 4 adult scallops from the control group and 12 from the treatment group at 8 months after fertilization. On average, after removing shared types of mutations, there were 1,151,380 +/- 258,188 SNPs (Single Nucleotide Polymorphisms) and 229,256 +/- 51,714 InDels (insertion-deletion) in each animal in the EMS treatment group, while there were only134841 +/- 10,115 SNPs and 42,605 +/- 5,136 InDels in the control group. The average mutation rate in the genome of the EMS treatment group (0.0137 +/- 0.0013%) was about 9 times that of the control group (0.0015 +/- 0.0002%). GO (Gene Ontology) annotation and KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analyses revealed that mutations induced by EMS occurred evenly in most biological processes, cellular components and functions, as well in most pathways. However, significant lower percentage of mutations were found in the exonic region, in non-synonymous or Stopgain/Stoploss SNPs and in coding domains, suggesting apparent DNA repair or selection during grow-out stage. Analyses of the growth-related genes with mutations indicated that mutations in MFS (Major Facilitator Superfamily) and Tubulin were only found in the large-sized group (Five largest scallops: Treated-1, Treated-2, Treated-3, Treated-4, and Treated-5) and Homeobox and Socs (Suppressor of cytokine signaling) only in the small group (Two smallest scallops: Treated-11 and Treated-12). These results suggested that these genes may be involved in the regulation of growth in these animals, although further verification is certainly warranted.Conclusion: Treatment of fertilized eggs with 20 mM EMS for 3 h induced 9 times more mutations in scallop genomes. We found that mutations in MFS and Tubulin may be related to fast growth in the large-sized group and those mutations in Homeobox and SOCs may be involved in the slow growth in the small-sized scallops. EMS can be used to accelerate selection of economically important traits in molluscs.
资助机构	Focally Deployed Program, Center for Ocean Mega-Science, Chinese Academy of Sciences ; National Natural Science Foundation ; Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province ; Earmarked Fund for Shandong Modern Agro Industry Technology Research System
收录类别	SCI
语种	英语
关键词[WOS]	MOLECULAR CHARACTERIZATION ; HOMEOBOX GENES ; SELECTION ; REVEALS ; DUPLICATION ; POPULATION ; EXPRESSION ; REGIONS
研究领域[WOS]	Genetics & Heredity
WOS记录号	WOS:000794899200001
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/37438
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Wang, Chunde
作者单位	1.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Wang, Caihui,Liu, Bo,Chen, Min,et al. Mutations in Growth-Related Genes Induced by EMS Treatment in Scallops[J]. FRONTIERS IN GENETICS,2022,13:13.
APA	Wang, Caihui,Liu, Bo,Chen, Min,Ning, Junhao,Lu, Xia,&Wang, Chunde.(2022).Mutations in Growth-Related Genes Induced by EMS Treatment in Scallops.FRONTIERS IN GENETICS,13,13.
MLA	Wang, Caihui,et al."Mutations in Growth-Related Genes Induced by EMS Treatment in Scallops".FRONTIERS IN GENETICS 13(2022):13.