Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum

doi:10.1016/j.fsi.2022.09.068

	Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum
	Lv, Xiaojing 1,2,6; Jiao, Chunli 3; Chen, Yuying 5; Chen, Lizhu 5; Li, Xuan 5; Liu, Xiangquan 5; Yang, Dinglong1,2,4 ; Zhao, Jianmin1,2,4
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2022-12-01
卷号	131 页码:77-83
关键词	Ruditapes philippinarum Histone Antimicrobial activity Extracellular traps
DOI	10.1016/j.fsi.2022.09.068
英文摘要	In the present study, a histone H2A (designed as RpH2A) was identified and characterized from clam Ruditapes philippinarum, and its open reading frame (ORF) was of 387 bp encoding a polypeptide of 128 amino acids. The deduced amino acid sequence of RpH2A shared high identities ranging from 57.1% to 96.1% with that of other identified H2A. The mRNA expression of RpH2A was up-regulated significantly after Vibrio anguillarum chal-lenge. The recombinant RpH2A protein (rRpH2A) displayed significantly binding affinity to lipopolysaccharide (LPS) and peptidoglycan (PGN) in vitro, and also exhibited antimicrobial properties against Escherichia coli. In addition, the antimicrobial RpH2A was shown to co-localize with extracellular traps (ETs) released from he-mocytes induced by E. coli, suggesting that RpH2A might contribute to eliminate invading bacteria in clam ETs. Altogether, our data revealed that RpH2A could function as antimicrobial peptides, which might play a crucial role in the immune responses of hemocytes ETs in clams.
收录类别	SCI
语种	英语
关键词[WOS]	MOLECULAR CHARACTERIZATION ; DISEASE VIRUS ; EXPRESSION ; HEMOCYTES ; IMMUNITY ; PROTEIN ; ANTIBODY ; H1
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000876937200001
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/37246
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Yang, Dinglong
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Peoples R China 3.Yantai Ctr Food & Drug Control, Yantai 264003, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China 5.Shandong Marine Resources & Environm Res Inst, Shandong Key Lab Marine Ecol Restorat, Yantai 264006, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lv, Xiaojing,Jiao, Chunli,Chen, Yuying,et al. Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2022,131:77-83.
APA	Lv, Xiaojing.,Jiao, Chunli.,Chen, Yuying.,Chen, Lizhu.,Li, Xuan.,...&Zhao, Jianmin.(2022).Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,131,77-83.
MLA	Lv, Xiaojing,et al."Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 131(2022):77-83.