Transcriptome analysis of hepatopancreas and gills of Palaemon gravieri under salinity stress

doi:10.1016/j.gene.2022.147013

	Transcriptome analysis of hepatopancreas and gills of Palaemon gravieri under salinity stress
	Shi, Wenjun 1,3; Hu, Runhao 1,2; Zhao, Ran 1,2; Zhu, Jianqiang 1,2; Shen, Hui 1; Li, Hui 1; Wang, Libao 1; Yang, Zeyu 4; Jiang, Qi 1; Qiao, Yi 1; Jiang, Ge 1; Cheng, Jie 1; Wan, Xihe 1,5
发表期刊	GENE
ISSN	0378-1119
	2023-01-30
卷号	851 页码:15
关键词	Palaemon gravieri Transcriptome Salinity stress Metabolism Transporters
DOI	10.1016/j.gene.2022.147013
通讯作者	Wan, Xihe([email protected])
英文摘要	Salinity is an important factor in the aquatic environment, and its fluctuations always result in osmotic stress, which affects the survival, distribution, and physiological activities of crustaceans. Crustaceans counter them through osmoregulation, which consists of many mechanisms. Palaemon gravieri is an important economic spe-cies in Palaemonidae, widely distributed in the southern East China Sea and the China Yellow Sea, and has a good adaptability to salinity stress. Currently, there are only a few studies on the effects of salinity on P. graviera. Therefore, it is particularly important to study the molecular responses of P. gravieri to salinity fluctuations. In this study, P. gravieri was treated with salinities of 10, 25, and 40, and the hepatopancreas and gills of shrimp in the different salinity groups were sampled after 24 h. The samples were used for RNA extraction and tran-scriptome analysis. In total, 80,994 unigenes were obtained, of which 19,114 were annotated. The differences in gene expression between different tissues at the same salinity were more significant. Many metabolism-related genes were downregulated in the gills, such as beta-hexosaminidase subunit alpha (HEXA), 10-formyltetrahydro-folate dehydrogenase (ALDH1L1), and Alcohol dehydrogenase class-3 (ADH5). Scanning transmission electron microscope analysis showed that the expression levels of some stress-(but not salinity stress) related genes changed after stress (mostly upregulated), suggesting the existence of secondary stress. Gene set enrichment analysis (GSEA) focused on the expression of transporters in osmoregulation, and the results showed that they mainly played a role in the gills, but ATP-binding cassette (ABC) transporters were more active in the hepato-pancreas. This study showed that the response of P. gravieri to salinity change was different not only between the hepatopancreas and gills, but also between low salinity and higher salinity, and the ion transport-related genes were mainly expressed in the gills. Overall, these results improve our understanding of salt tolerance mechanism in P. gravieri.
资助机构	Jiangsu Provincial Agricultural Major New Varieties Creation Project ; Jiangsu Agricultural Industry Technology System ; Project of Seed Industry Revitalization in Jiangsu Province ; Jiangsu Agricultural Science and Technology Innovation Fund
收录类别	SCI
语种	英语
关键词[WOS]	HIGH-DENSITY-LIPOPROTEIN ; PACIFIC WHITE SHRIMP ; H+-ATPASE ; LITOPENAEUS-VANNAMEI ; OSMOREGULATORY CAPACITY ; ALCOHOL-DEHYDROGENASE ; MOLECULAR-CLONING ; QUANTITATIVE PCR ; ANION CHANNELS ; NA+/K+-ATPASE
研究领域[WOS]	Genetics & Heredity
WOS记录号	WOS:000883040500005
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/37192
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Wan, Xihe
作者单位	1.Inst Oceanol & Marine Fisheries, Nantong 226007, Jiangsu, Peoples R China 2.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 4.Aquat Prod Technol Extens Stn Gaoyou, Gaoyou 225634, Peoples R China 5.Inst Oceanol & Marine Fisheries, 31 Jiaoyu Rd, Nantong 226007, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Shi, Wenjun,Hu, Runhao,Zhao, Ran,et al. Transcriptome analysis of hepatopancreas and gills of Palaemon gravieri under salinity stress[J]. GENE,2023,851:15.
APA	Shi, Wenjun.,Hu, Runhao.,Zhao, Ran.,Zhu, Jianqiang.,Shen, Hui.,...&Wan, Xihe.(2023).Transcriptome analysis of hepatopancreas and gills of Palaemon gravieri under salinity stress.GENE,851,15.
MLA	Shi, Wenjun,et al."Transcriptome analysis of hepatopancreas and gills of Palaemon gravieri under salinity stress".GENE 851(2023):15.