Nonylphenol displays immunotoxicity by triggering hemocyte extracellular traps in Manila clam via ROS burst, ERK pathway and glycolysis

doi:10.1016/j.ecoenv.2024.117145

	Nonylphenol displays immunotoxicity by triggering hemocyte extracellular traps in Manila clam via ROS burst, ERK pathway and glycolysis
	Lv, Xiaojing 1,3,4; Han, Yijing 5; Li, Yongxue 1,3; Wang, Xin 1,3; Zhang, Tianyu 1,3; Wang, Xiaodan 1,3; Zhang, Qianqian1,3 ; Yang, Dinglong1,2,3 ; Zhao, Jianmin1,2,3
发表期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
	2024-10-15
卷号	285 页码:14
关键词	Nonylphenol Extracellular traps ERK signaling pathway Reactive oxygen species Glycolysis
DOI	10.1016/j.ecoenv.2024.117145
通讯作者	Yang, Dinglong([email protected]) ; Zhao, Jianmin([email protected])
英文摘要	Nonylphenol (NP), an endocrine disruptor, has been demonstrated to be a harmful environmental contaminant and toxic to organisms. In this study, to address concerns regarding the immunotoxicity of NP, we treated clam Ruditapes philippinarum hemocytes with NP in vitro and explored the underlying mechanisms of NP-induced extracellular traps (ETs). NP could induce the formation of hemocytes ETs in a dose-dependent manner. Transcriptomics analysis revealed changes of signaling pathway involved in immunity and energy metabolism in hemocytes after NP stimulation. In this process, both reactive oxygen species (ROS) and myeloperoxidase (MPO) were up-regulated. Moreover, mitogen-activated protein kinase (MAPK) signaling pathway was proved to be activated in the formation of NP-induced ETs, manifested as enhanced phosphorylation of extracellular signal-regulated kinase (ERK) but not p38 or c-Jun N-terminal kinase (JNK). In the presence of U0126, an ERK phosphorylation inhibitor, the NP-induced expression of NADPH oxidase enzyme (NOX) was significantly decreased, which further alleviated the ROS production and ultimately limited the release of ETs. NP exposure increased glucose uptake, along with enhanced activities of glycolysis-related enzymes such as hexokinase (HK) and pyruvate kinase (PK). After inhibiting glycolysis by the inhibitor 2-DG, the formation of NP-induced ETs was significantly suppressed. ERK could regulate mTOR signaling and the PI3K/AKT pathway, potentially directing ETs formation by orchestrating the glycolysis through the activation of key transcription factors c-Myc and HIF-1 alpha. Collectively, the results preliminary confirm that the ERK-NOX-ROS axis and glycolysis are involved in NP-induced ETs formation, contributing to the cellular immunotoxicity in clam.
资助机构	Shandong Key Laboratory of Marine Ecological Restoration ; National Science & Technology Fundamental Resources Investigation Program of China ; Seed Project of Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences
收录类别	SCI
语种	英语
关键词[WOS]	OYSTER CRASSOSTREA-GIGAS ; PARTICULATE MATTER ; MARINE ORGANISMS ; BISPHENOL-A ; 4-NONYLPHENOL ; RELEASE ; IMMUNITY ; EXPOSURE ; FATE ; IMMUNOMODULATION
研究领域[WOS]	Environmental Sciences & Ecology ; Toxicology
WOS记录号	WOS:001330943500001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/37144
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Yang, Dinglong; Zhao, Jianmin
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Ludong Univ, Sch Fisheries, Yantai 264025, Peoples R China
推荐引用方式 GB/T 7714	Lv, Xiaojing,Han, Yijing,Li, Yongxue,et al. Nonylphenol displays immunotoxicity by triggering hemocyte extracellular traps in Manila clam via ROS burst, ERK pathway and glycolysis[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2024,285:14.
APA	Lv, Xiaojing.,Han, Yijing.,Li, Yongxue.,Wang, Xin.,Zhang, Tianyu.,...&Zhao, Jianmin.(2024).Nonylphenol displays immunotoxicity by triggering hemocyte extracellular traps in Manila clam via ROS burst, ERK pathway and glycolysis.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,285,14.
MLA	Lv, Xiaojing,et al."Nonylphenol displays immunotoxicity by triggering hemocyte extracellular traps in Manila clam via ROS burst, ERK pathway and glycolysis".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 285(2024):14.