Single-cell RNA sequencing revealed the roles of macromolecule epidermal growth factor receptor (<i>EGFR</i>) in the hybrid sterility of hermaphroditic <i>Argopecten</i> scallops

doi:10.1016/j.ijbiomac.2024.136062

	*Single-cell RNA sequencing revealed the roles of macromolecule epidermal growth factor receptor (EGFR) in the hybrid sterility of hermaphroditic Argopecten* scallops**
	Yu, Tieying 1,4; Wang, Chunde 1,3; Fan, Jiawei 1,4; Chen, Rongjie 5; Liu, Guilong 6; Xu, Xin 6; Ning, Junhao 1; Lu, Xia2
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
	2024-11-01
卷号	280 页码:13
关键词	Epidermal growth factor receptor Hybrid sterility Argopecten scallops ATP ROS
DOI	10.1016/j.ijbiomac.2024.136062
通讯作者	Ning, Junhao([email protected]) ; Lu, Xia([email protected])
英文摘要	The macromolecule epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein that belongs to the protein kinase superfamily, which plays versatile functions in cell proliferation, development and fertility regulation. Almost all F1 hybrids obtained from the hermaphroditic bay scallops and Peruvian scallops exhibit infertility, and the genetic mechanism remains unclear. In this study, the comprehensive scRNA-seq was first conducted in the gonads of hybrid scallops, deducing the developmental sequence of germ cells and identifying the critical regulators in hybrid sterility: epidermal growth factor receptor. During the development from oogenesis phase germ cells to oocytes, the expression of the EGFR gene gradually decreased in sterile hybrids but increased in fertile hybrids. The significantly lower EGFR expression and ATP content, but higher ROS production rate was detected in the gonad of sterile hybrids than that in fertile hybrids, which might cause slow development of oocytes, stagnation of cell cycle, insufficient energy supply, high level of apoptosis and final sterility. Specific knock-down of EGFR gene led to decreased ATP content, increased ROS production rate, and inhibited oocyte maturation and gonadal development. These findings provide new insights into the roles of EGFR in hybrid infertility of bivalves and the healthy development of scallop breeding.
资助机构	National Natural Science Foundation of China ; Scientific and Technological Project of Yantai, Shandong Province ; Earmarked Fund for Shandong Modern Agro-Industry Technology Research System
收录类别	SCI
语种	英语
关键词[WOS]	BAY SCALLOP ; ACTIVATION ; DIFFERENTIATION ; EXPRESSION ; EGGSHELL ; EMBRYOS ; GENE
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS记录号	WOS:001329498600001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/37136
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Ning, Junhao; Lu, Xia
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Shandong, Peoples R China 2.Yantai Univ, Sch Ocean, Yantai 264005, Shandong, Peoples R China 3.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Laizhou Marine Dev & Fishery Serv Ctr, Laizhou 261400, Peoples R China 6.Yantai Spring Sea AquaSeed Ltd, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Yu, Tieying,Wang, Chunde,Fan, Jiawei,et al. Single-cell RNA sequencing revealed the roles of macromolecule epidermal growth factor receptor (EGFR) in the hybrid sterility of hermaphroditic Argopecten scallops[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,280:13.
APA	Yu, Tieying.,Wang, Chunde.,Fan, Jiawei.,Chen, Rongjie.,Liu, Guilong.,...&Lu, Xia.(2024).Single-cell RNA sequencing revealed the roles of macromolecule epidermal growth factor receptor (EGFR) in the hybrid sterility of hermaphroditic Argopecten scallops.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,280,13.
MLA	Yu, Tieying,et al."Single-cell RNA sequencing revealed the roles of macromolecule epidermal growth factor receptor (EGFR) in the hybrid sterility of hermaphroditic Argopecten scallops".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 280(2024):13.