Tetrahedral DNA framework assisted rotational paper-based analytical device for differential detection of SARS-CoV-2 and influenza A H1N1 virus

doi:10.1016/j.microc.2022.108304

	Tetrahedral DNA framework assisted rotational paper-based analytical device for differential detection of SARS-CoV-2 and influenza A H1N1 virus
	Li, Fengling 1; Qi, Ji 3; Ren, Zengzheng 1; Hu, Xiaoli 1; Chen, Yan 2; Li, Bowei3 ; Fu, Xiuli 1
发表期刊	MICROCHEMICAL JOURNAL
ISSN	0026-265X
	2023-02-01
卷号	185 页码:7
关键词	Tetrahedral DNA framework Paper-based analytical device SARS-CoV-2
DOI	10.1016/j.microc.2022.108304
通讯作者	Chen, Yan([email protected]) ; Li, Bowei([email protected]) ; Fu, Xiuli([email protected])
英文摘要	Coronavirus disease 2019 (COVID-19) and influenza A are two respiratory infectious diseases with similar clinical manifestations. Because of the complex global epidemic situation of COVID-19, the distinction and diagnosis of COVID-19 and influenza A infected persons is crucial for epidemic prevention and control. In this study, tetrahedral DNA framework (TDF) was combined with a rotational paper-based analytical device, and the color change generated by the reaction between horseradish peroxidase (HRP) and 3,3 ' 5,5 '-tetramethylbenzidine (TMB)-H2O2 was used for grayscale signal analysis by ImageJ software. The quantitative detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza A H1N1 virus were realized simulta-neously. Under the optimal conditions, the paper-based analytical device showed a good linear relationship between the two viruses in the range of 10-14-10-8g/mL, and the two viruses were not affected by cross reaction. This sensor provides a convenient and reliable method for clinical rapid differentiation and diagnosis of COVID-19 and influenza A.
资助机构	Qingchuang Science and Technology Program of Shandong Province in China ; National Natural Science Foundation of China ; Shandong Provincial Natural Science Foundation Key Project
收录类别	SCI
语种	英语
关键词[WOS]	NANOSTRUCTURE ; MICROFLUIDICS ; PLATFORM
研究领域[WOS]	Chemistry
WOS记录号	WOS:000905569200001
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/37109
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
通讯作者	Chen, Yan; Li, Bowei; Fu, Xiuli
作者单位	1.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China 2.Shandong Agr & Engn Univ, Sch Resources & Environm Engn, Jinan 250100, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Li, Fengling,Qi, Ji,Ren, Zengzheng,et al. Tetrahedral DNA framework assisted rotational paper-based analytical device for differential detection of SARS-CoV-2 and influenza A H1N1 virus[J]. MICROCHEMICAL JOURNAL,2023,185:7.
APA	Li, Fengling.,Qi, Ji.,Ren, Zengzheng.,Hu, Xiaoli.,Chen, Yan.,...&Fu, Xiuli.(2023).Tetrahedral DNA framework assisted rotational paper-based analytical device for differential detection of SARS-CoV-2 and influenza A H1N1 virus.MICROCHEMICAL JOURNAL,185,7.
MLA	Li, Fengling,et al."Tetrahedral DNA framework assisted rotational paper-based analytical device for differential detection of SARS-CoV-2 and influenza A H1N1 virus".MICROCHEMICAL JOURNAL 185(2023):7.