Phylogenetic Analysis and Characterization of Diguanylate Cyclase and Phosphodiesterase in Planktonic Filamentous Cyanobacterium <i>Arthrospira</i> sp.

doi:10.3390/ijms242015210

	*Phylogenetic Analysis and Characterization of Diguanylate Cyclase and Phosphodiesterase in Planktonic Filamentous Cyanobacterium Arthrospira* sp.**
	Wang, Kang 1,2; Li, Wenjun1 ; Cui, Hongli1 ; Qin, Song1
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN	1661-6596
	2023-10-01
卷号	24 期号:20 页码:11
关键词	c-di-GMP Arthrospira diguanylate cyclase phosphodiesterase riboswitch
DOI	10.3390/ijms242015210
通讯作者	Qin, Song([email protected])
英文摘要	Cyclic di-GMP (c-di-GMP) is a second messenger of intracellular communication in bacterial species, which widely modulates diverse cellular processes. However, little is known about the c-di-GMP network in filamentous multicellular cyanobacteria. In this study, we preliminarily investigated the c-di-GMP turnover proteins in Arthrospira based on published protein data. Bioinformatics results indicate the presence of at least 149 potential turnover proteins in five Arthrospira subspecies. Some proteins are highly conserved in all tested Arthrospira, whereas others are specifically found only in certain subspecies. To further validate the protein catalytic activity, we constructed a riboswitch-based c-di-GMP expression assay system in Escherichia coli and confirmed that a GGDEF domain protein, Adc11, exhibits potential diguanylate cyclase activity. Moreover, we also evaluated a protein with a conserved HD-GYP domain, Ahd1, the expression of which significantly improved the swimming ability of E. coli. Enzyme-linked immunosorbent assay also showed that overexpression of Ahd1 reduced the intracellular concentration of c-di-GMP, which is presumed to exhibit phosphodiesterase activity. Notably, meta-analyses of transcriptomes suggest that Adc11 and Ahd1 are invariable. Overall, this work confirms the possible existence of a functional c-di-GMP network in Arthrospira, which will provide support for the revelation of the biological function of the c-di-GMP system in Arthrospira.
资助机构	High-tech industrialization special project in cooperation between Jilin Province ; Chinese Academy of Sciences ; Major Science and Technology Innovation Project of Shandong Province ; Natural Science Foundation of Shandong Province
收录类别	SCI
语种	英语
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
WOS记录号	WOS:001099386200001
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/36667
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Qin, Song
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Kang,Li, Wenjun,Cui, Hongli,et al. Phylogenetic Analysis and Characterization of Diguanylate Cyclase and Phosphodiesterase in Planktonic Filamentous Cyanobacterium Arthrospira sp.[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2023,24(20):11.
APA	Wang, Kang,Li, Wenjun,Cui, Hongli,&Qin, Song.(2023).Phylogenetic Analysis and Characterization of Diguanylate Cyclase and Phosphodiesterase in Planktonic Filamentous Cyanobacterium Arthrospira sp..INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,24(20),11.
MLA	Wang, Kang,et al."Phylogenetic Analysis and Characterization of Diguanylate Cyclase and Phosphodiesterase in Planktonic Filamentous Cyanobacterium Arthrospira sp.".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24.20(2023):11.