Lipid accumulation mechanism of <i>Amphora coffeaeformis</i> under nitrogen deprivation and its application as a feed additive in <i>Carassius auratus</i> aquaculture

doi:10.1186/s13068-023-02436-7

	*Lipid accumulation mechanism of Amphora coffeaeformis* under nitrogen deprivation and its application as a feed additive in Carassius auratus aquaculture**
	Cui, Yulin1 ; Wang, Kang 2,3; Zhou, Xiuzhi 1; Meng, Chunxiao 1; Gao, Zhengquan 1
发表期刊	BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS
	2023-12-06
卷号	16 期号:1 页码:10
关键词	Amphora coffeaeformis Nitrogen deprivation Lipid accumulation RNA-seq TAG Crucian carp Fatty acids
DOI	10.1186/s13068-023-02436-7
通讯作者	Gao, Zhengquan([email protected])
英文摘要	Background Amphora coffeaeformis, a unicellular diatom, can significantly accumulate lipids under nitrogen (N) limitation. However, the molecular mechanism underlying lipid accumulation in A. coffeaeformis remains unknown and its application development is lagging.Results This work analyzed the lipid composition of A. coffeaeformis under N deprivation and investigated its mechanism underlying lipid accumulation using RNA-seq. The results showed that the total lipid content of A. coffeaeformis increased from 28.22 to 44.05% after 5 days of N deprivation, while the neutral lipid triacylglycerol (TAG) content increased from 10.41 to 25.21%. The transcriptional profile showed that N deprivation induced wide-ranging reprogramming of regulation and that most physiological activities were repressed, while the upregulation of glycerol-3-phosphate acyltransferase directly determined TAG accumulation. Moreover, we explored the effect of A. coffeaeformis as a food additive on the lipid composition of crucian carp. The results showed that the contents of unsaturated fatty acids in the meat of fish supplemented with A. coffeaeformis were significantly increased, indicating its potential application in animal nutrition for improving meat quality indicators.Conclusion The findings shed light on the molecular mechanisms of neutral lipid accumulation and revealed the key genes involved in lipid metabolism in A. coffeaeformis. Moreover, we also confirmed that A. coffeaeformis can be used as feed additive for improving the lipid composition of crucian carp meat, which provided evidence for the biotechnology application of this high-oil microalgae.
资助机构	National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Binzhou Medical University
收录类别	SCI
语种	英语
关键词[WOS]	MICROALGAL BIOMASS ; CULTIVATION ; METABOLISM ; GROWTH ; ALGAE
研究领域[WOS]	Biotechnology & Applied Microbiology ; Energy & Fuels
WOS记录号	WOS:001115252300001
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/36593
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Gao, Zhengquan
作者单位	1.Binzhou Med Univ, Dept Physiol, 346 Guanhai Rd, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 101418, Peoples R China
推荐引用方式 GB/T 7714	Cui, Yulin,Wang, Kang,Zhou, Xiuzhi,et al. Lipid accumulation mechanism of Amphora coffeaeformis under nitrogen deprivation and its application as a feed additive in Carassius auratus aquaculture[J]. BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS,2023,16(1):10.
APA	Cui, Yulin,Wang, Kang,Zhou, Xiuzhi,Meng, Chunxiao,&Gao, Zhengquan.(2023).Lipid accumulation mechanism of Amphora coffeaeformis under nitrogen deprivation and its application as a feed additive in Carassius auratus aquaculture.BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS,16(1),10.
MLA	Cui, Yulin,et al."Lipid accumulation mechanism of Amphora coffeaeformis under nitrogen deprivation and its application as a feed additive in Carassius auratus aquaculture".BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS 16.1(2023):10.