Structural framework for the understanding spectroscopic and functional signatures of the cyanobacterial Orange Carotenoid Protein families

doi:10.1016/j.ijbiomac.2023.127874

	Structural framework for the understanding spectroscopic and functional signatures of the cyanobacterial Orange Carotenoid Protein families
	Sluchanko, Nikolai N.1; Maksimov, Eugene G.2; Slonimskiy, Yury B.1; Varfolomeeva, Larisa A.1; Bukhanko, Antonina Y.2; Egorkin, Nikita A.1,2; Tsoraev, Georgy, V 1; Khrenova, Maria G.1,3; Ge, Baosheng 4; Qin, Song5 ; Boyko, Konstantin M.1; Popov, Vladimir O.1,2
发表期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN	0141-8130
	2024
卷号	254 页码:20
关键词	Photoprotection Phycobilisome fluorescence quenching Crystal structure Protein-protein interaction Absorption spectroscopy Photoactivity SEC-MALS
DOI	10.1016/j.ijbiomac.2023.127874
通讯作者	Sluchanko, Nikolai N.([email protected]) ; Qin, Song([email protected])
英文摘要	The Orange Carotenoid Protein (OCP) is a unique photoreceptor crucial for cyanobacterial photoprotection. Best studied Synechocystis sp. PCC 6803 OCP belongs to the large OCP1 family. Downregulated by the Fluorescence Recovery Protein (FRP) in low-light, high-light-activated OCP1 binds to the phycobilisomes and performs non -photochemical quenching. Recently discovered families OCP2 and OCP3 remain structurally and functionally underexplored, and no systematic comparative studies have ever been conducted. Here we present two first crystal structures of OCP2 from morphoecophysiologically different cyanobacteria and provide their comprehensive structural, spectroscopic and functional comparison with OCP1, the recently described OCP3 and all-OCP ancestor. Structures enable correlation of spectroscopic signatures with the effective number of hydrogen and discovered here chalcogen bonds anchoring the ketocarotenoid in OCP, as well as with the rotation of the echinenone's beta-ionone ring in the CTD. Structural data also helped rationalize the observed differences in OCP/ FRP and OCP/phycobilisome functional interactions. These data are expected to foster OCP research and applications in optogenetics, targeted carotenoid delivery and cyanobacterial biomass engineering.
资助机构	Program of the Ministry of Science and Higher Education of the Russian Federation ; joint Russian Science Foundation/National Scientific Fund of China ; Program of the Ministry of Science and Higher Education of the Russian Federation ; Russian Science Foundation/National Scientific Fund of China
收录类别	SCI
语种	英语
关键词[WOS]	N-TERMINAL DOMAIN ; PHOTOPROTECTIVE MECHANISM ; CRYSTAL-STRUCTURE ; SIGNALING STATE ; RECOVERY ; IDENTIFICATION ; TRANSLOCATION ; FLUORESCENCE ; MUTANT ; COLOR
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS记录号	WOS:001113594000001
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/36583
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Sluchanko, Nikolai N.; Qin, Song
作者单位	1.AN Bach Inst Biochem, Fed Res Ctr Biotechnol Russian Acad Sci, AN Bach Inst Biochem, Moscow 119071, Russia 2.Moscow MV Lomonosov State Univ, Fac Biol, Moscow 119991, Russia 3.Lomonosov Moscow State Univ, Chem Dept, Moscow 119991, Russia 4.China Univ Petr Huadong, Coll Chem & Chem Engn, Qingdao 266580, Peoples R China 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Sluchanko, Nikolai N.,Maksimov, Eugene G.,Slonimskiy, Yury B.,et al. Structural framework for the understanding spectroscopic and functional signatures of the cyanobacterial Orange Carotenoid Protein families[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,254:20.
APA	Sluchanko, Nikolai N..,Maksimov, Eugene G..,Slonimskiy, Yury B..,Varfolomeeva, Larisa A..,Bukhanko, Antonina Y..,...&Popov, Vladimir O..(2024).Structural framework for the understanding spectroscopic and functional signatures of the cyanobacterial Orange Carotenoid Protein families.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,254,20.
MLA	Sluchanko, Nikolai N.,et al."Structural framework for the understanding spectroscopic and functional signatures of the cyanobacterial Orange Carotenoid Protein families".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 254(2024):20.