CsIL-20, a tongue sole interleukin-20, negatively mediates leucocyte activity and antibacterial defense

doi:10.1016/j.fsi.2023.109325

	CsIL-20, a tongue sole interleukin-20, negatively mediates leucocyte activity and antibacterial defense
	Zhao, Kun-yu 1; Huang, Hui 2; Jin, Qiu 1; Jiao, Xu-dong3,5 ; Li, Xue-peng 1,4
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2024-02-01
卷号	145 页码:9
关键词	Interleukin-20 Cynoglossus semilaevis Expression patterns PBLs activity Antibacterial immunity
DOI	10.1016/j.fsi.2023.109325
通讯作者	Jiao, Xu-dong([email protected]) ; Li, Xue-peng([email protected])
英文摘要	Interleukin-20 (IL-20), as an essential member of IL-10 family, plays vital roles in mammalian immunological response such as antimicrobial, inflammation, hematopoiesis, and immune diseases. In teleost, the study about immune antimicrobial function of IL-20 is largely scarce. In this article, we revealed the expression profiles and the immunological functions of the IL-20 (CsIL-20) in tongue sole Cynoglossus semilaevis. CsIL-20 is composed of 183 amino acid residues, with seven cysteine residues and a typical IL-10 domain which comprises six alpha-helices and two 13-sheets, and shares 34.4-71.2 % identities with other teleost IL-20. CsIL-20 was constitutively expressed in a variety of tissues and regulated by bacterial invasion, and the recombinant CsIL-20 (rCsIL-20) could bind to different bacteria. In vitro rCsIL-20 could interact with the membrane of peripheral blood leukocytes (PBLs), leading to the attenuation of reactive oxygen species (ROS) production and acid phosphatase activity in PBLs. In line with In vitro results, In vivo rCsIL-20 could obviously suppressed the host immune against bacterial infection. Furthermore, knockdown of CsIL-20 in vivo could markedly enhance the host antibacterial immunity. Collectively, these observations offer new insights into the negative effect of CsIL-20 on antibacterial immunity.
资助机构	Development Plan of Youth Innovation Team in Colleges and Universities of Shandong Province ; Yantai Key Laboratory of Quality and Safety Control and Deep Processing of Marine Food ; Shandong Provincial Modern Agricultural Industry Technology System
收录类别	SCI
语种	英语
关键词[WOS]	BACTERIAL-INFECTION ; RECEPTOR COMPLEXES ; IL-20 SUBFAMILY ; IL-10 FAMILY ; IN-VITRO ; CYTOKINES ; ACTIVATION ; IL-1-BETA ; IMMUNITY ; PATHWAY
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:001154541100001
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/36355
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Jiao, Xu-dong; Li, Xue-peng
作者单位	1.Yantai Univ, Sch Ocean, Yantai, Peoples R China 2.Shandong Marine Resource & Environm Res Inst, Yantai, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai, Peoples R China 4.Yantai Univ, 30 Qingquan Rd, Yantai 264005, Peoples R China 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Kun-yu,Huang, Hui,Jin, Qiu,et al. CsIL-20, a tongue sole interleukin-20, negatively mediates leucocyte activity and antibacterial defense[J]. FISH & SHELLFISH IMMUNOLOGY,2024,145:9.
APA	Zhao, Kun-yu,Huang, Hui,Jin, Qiu,Jiao, Xu-dong,&Li, Xue-peng.(2024).CsIL-20, a tongue sole interleukin-20, negatively mediates leucocyte activity and antibacterial defense.FISH & SHELLFISH IMMUNOLOGY,145,9.
MLA	Zhao, Kun-yu,et al."CsIL-20, a tongue sole interleukin-20, negatively mediates leucocyte activity and antibacterial defense".FISH & SHELLFISH IMMUNOLOGY 145(2024):9.