Sea Cucumber Viscera Contains Novel Non-Holostane-Type Glycoside Toxins that Possess a Putative Chemical Defense Function

doi:10.1007/s10886-024-01483-0

	Sea Cucumber Viscera Contains Novel Non-Holostane-Type Glycoside Toxins that Possess a Putative Chemical Defense Function
	Liu, Yanfang 1,3; Lu, Zhen 1; Yan, Zhi 1,2; Lin, Ainuo 1,3; Han, Shaoshuai 1,4; Li, Yaxi 1,3; Yang, Xiao 1,3; Li, Xiaodong 1; Yin, Xiuli1 ; Zhang, Ranran 1,3; Li, Ke1
发表期刊	JOURNAL OF CHEMICAL ECOLOGY
ISSN	0098-0331
	2024-03-05
页码	12
关键词	Saponin Viscera Non-holostane Acute toxicity Natural products Chemical defense
DOI	10.1007/s10886-024-01483-0
通讯作者	Li, Ke([email protected])
英文摘要	Sea cucumbers frequently expel their guts in response to predators and an aversive environment, a behavior perceived as releasing repellents involved in chemical defense mechanisms. To investigate the chemical nature of the repellent, the viscera of stressed sea cucumbers (Apostichopus japonicus) in the Yellow Sea of China were collected and chemically analyzed. Two novel non-holostane triterpene glycosides were isolated, and the chemical structures were elucidated as 3 beta-O-[beta-D-glucopyranosyl-(1 -> 2)-beta-D-xylopyranosyl]-(20S)-hydroxylanosta-7,25-diene-18(16)-lactone (1) and 3 beta-O-[beta-D-quinovopyranosyl-(1 -> 2)-beta-D-xylopyranosyl]-(20S)-hydroxylanosta-7,25-diene-18(16)-lactone (2) by spectroscopic and mass-spectrometric analyses, exemplifying a triterpene glycoside constituent of an oligosaccharide containing two sugar-units and a non-holostane aglycone. Zebrafish embryos were exposed to various doses of 1 and 2 from 4 to 96 hpf. Compound 1 exposure showed 96 h-LC50 41.5 mu M and an increased zebrafish mortality rates in roughly in a dose- and time-dependent manner. Compound 2, with different sugar substitution, exhibited no mortality and moderate teratogenic toxicity with a 96 h-EC50 of 173.5 mu M. Zebrafish embryos exhibited teratogenic effects, such as reduced hatchability and total body length. The study found that triterpene saponin from A. japonicus viscera had acute toxicity in zebrafish embryos, indicating a potential chemical defense role in the marine ecosystem.
资助机构	National Natural Science Foundation of China
收录类别	SCI
语种	英语
关键词[WOS]	TRITERPENE GLYCOSIDES ; CUVIERIAN TUBULES ; SAPONINS ; ZEBRAFISH ; HOLOTHURIANS ; EFFICIENCY ; MODEL
研究领域[WOS]	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology
WOS记录号	WOS:001176417100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/36171
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Li, Ke
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Yantai Univ, Sch Ocean, Yantai 264005, Peoples R China 3.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 4.Harbin Inst Technol, Sch Marine Sci & Technol, Weihai 264209, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yanfang,Lu, Zhen,Yan, Zhi,et al. Sea Cucumber Viscera Contains Novel Non-Holostane-Type Glycoside Toxins that Possess a Putative Chemical Defense Function[J]. JOURNAL OF CHEMICAL ECOLOGY,2024:12.
APA	Liu, Yanfang.,Lu, Zhen.,Yan, Zhi.,Lin, Ainuo.,Han, Shaoshuai.,...&Li, Ke.(2024).Sea Cucumber Viscera Contains Novel Non-Holostane-Type Glycoside Toxins that Possess a Putative Chemical Defense Function.JOURNAL OF CHEMICAL ECOLOGY,12.
MLA	Liu, Yanfang,et al."Sea Cucumber Viscera Contains Novel Non-Holostane-Type Glycoside Toxins that Possess a Putative Chemical Defense Function".JOURNAL OF CHEMICAL ECOLOGY (2024):12.