Salinity Acclimation Induces Reduced Energy Metabolism, Osmotic Pressure Regulation and Transcriptional Reprogramming in Hypotrichida Ciliate <i>Gastrostyla setifera</i>

doi:10.1007/s11802-024-5714-x

	Salinity Acclimation Induces Reduced Energy Metabolism, Osmotic Pressure Regulation and Transcriptional Reprogramming in Hypotrichida Ciliate Gastrostyla setifera
	Ji, Xin 1; Bi, Luping 2; Zou, Songbao 3; Li, Wenlu 1; Ji, Daode 1; Zhang, Qianqian2,4
发表期刊	JOURNAL OF OCEAN UNIVERSITY OF CHINA
ISSN	1672-5182
	2024-04-01
卷号	23 期号:2 页码:539-549
关键词	salinity stress heterotrophic protist ciliate Gastrostyla setifera transcriptome differentially expressed gene
DOI	10.1007/s11802-024-5714-x
通讯作者	Ji, Daode([email protected]) ; Zhang, Qianqian([email protected])
英文摘要	Coastal and estuarine protists are frequently exposed to salinity undulation. While the tolerance and stress responses of microalgae to salinity have been extensively studied, there have been scarce studies on the physiological response of heterotrophic protists to salinity stressing. In this study, we investigated the physiological response of the heterotrophic ciliate Gastrostyla setifera to a salinity of 3, via a transcriptomic approach. The first transcriptome of genus Gastrostyla was obtained utilizing a group of manually isolated ciliate individuals (cells) and RNA-seq technique. The completeness of the transcriptome was verified. Differentially expressed gene (DEG) analysis was performed among the transcriptomes of G setifera acclimated in saline water (salinity 3) and those cultured in fresh water. The results demonstrated a significant alternation in gene transcription, in which the ciliate exhibits a transcripttomic acclimation in responding salinity stressing. The up-regulated DEGs were enriched in the pathways of cytoskeleton proteins, membrane trafficking, protein kinases and protein phosphatases. These may represent enhanced functions of ion transport, stress response and cell protections. Pathways involved in energy metabolism and biosynthesis were markedly down-regulated, reflecting decreased cell activity. Particularly, we detected significantly down-regulated genes involved in several pathways of amino acid catabolism, which may lead to accumulation of amino acids in the ciliate cell. Amino acid could act as compatible solutes in the cytoplasm to maintain the osmotic balance in saline water. Overall, this work is an initial exploration to the molecular basis of the heterotrophic protist responding to salinity stressing. The result sheds light on the mechanisms of enhancement of cell protection, reduction of cell activity, and osmotic pressure regulation in ciliates acclimated to salinity.
资助机构	National Natural Science Foundation of China ; Youth Innovation Promotion Association of CAS
收录类别	SCI
语种	英语
关键词[WOS]	STRESS ; CILIOPHORA ; PHYLOGENY ; TOLERANCE ; PATTERNS ; GENES
研究领域[WOS]	Oceanography
WOS记录号	WOS:001195787500022
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/36123
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Ji, Daode; Zhang, Qianqian
作者单位	1.Yantai Univ, Sch Ocean, Yantai 264005, Peoples R China 2.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Zhejiang Inst Freshwater Fisheries, Huzhou 313001, Peoples R China 4.Chinese Acad Sci, Muping Coastal Environm Res Stn, Yantai Inst Coastal Zone Res, Yantai 264117, Peoples R China
推荐引用方式 GB/T 7714	Ji, Xin,Bi, Luping,Zou, Songbao,et al. Salinity Acclimation Induces Reduced Energy Metabolism, Osmotic Pressure Regulation and Transcriptional Reprogramming in Hypotrichida Ciliate Gastrostyla setifera[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2024,23(2):539-549.
APA	Ji, Xin,Bi, Luping,Zou, Songbao,Li, Wenlu,Ji, Daode,&Zhang, Qianqian.(2024).Salinity Acclimation Induces Reduced Energy Metabolism, Osmotic Pressure Regulation and Transcriptional Reprogramming in Hypotrichida Ciliate Gastrostyla setifera.JOURNAL OF OCEAN UNIVERSITY OF CHINA,23(2),539-549.
MLA	Ji, Xin,et al."Salinity Acclimation Induces Reduced Energy Metabolism, Osmotic Pressure Regulation and Transcriptional Reprogramming in Hypotrichida Ciliate Gastrostyla setifera".JOURNAL OF OCEAN UNIVERSITY OF CHINA 23.2(2024):539-549.