Chronopotentiometric sensors for antimicrobial peptide-based biosensing of <i>Staphylococcus aureus</i>

doi:10.1007/s00604-024-06410-4

	Chronopotentiometric sensors for antimicrobial peptide-based biosensing of Staphylococcus aureus
	Zhao, Jiarong 1,2; Ding, Jiawang2 ; Luan, Feng 1; Qin, Wei2,3,4
发表期刊	MICROCHIMICA ACTA
ISSN	0026-3672
	2024-06-01
卷号	191 期号:6 页码:11
关键词	Chronopotentiometry Ion-selective electrode Peptide Biosensing Bacteria
DOI	10.1007/s00604-024-06410-4
通讯作者	Ding, Jiawang([email protected])
英文摘要	Charged antimicrobial peptides can be used for direct potentiometric biosensing, but have never been explored. We report here a galvanostatically-controlled potentiometric sensor for antimicrobial peptide-based biosensing. Solid-state pulsed galvanostatic sensors that showed excellent stability under continuous galvanostatic polarization were prepared by utilizing reduced graphene oxide/poly (3,4-ethylenedioxythiophene): poly (4-styrenesulfonate) (rGO/PEDOT: PSS) as a solid contact. More importantly, the chronopotentiometric sensor can be made sensitive to antimicrobial peptides with intrinsic charge on demand via a current pulse. In this study, a positively charged antimicrobial peptide that can bind to Staphylococcus aureus with high affinity and good selectivity was designed as a model. Two arginine residues with positive charges were linked to the C-terminal of the peptide sequence to increase its potentiometric responses on the electrode. The bacteria binding-induced charge or charge density change of the antimicrobial peptide enables the direct chronopotentiometric detection of the target. Under the optimized conditions, the concentration of Staphylococcus aureus can be determined in the linear range 10-1.0 x 10(5) CFU mL(-1) with a detection limit of 10 CFU mL(-1). It is anticipated that such a chronopotentiometric sensing platform is readily adaptable to detect other bacteria by choosing the peptides.
资助机构	National Natural Science Foundation of China ; Taishan Scholar Program of Shandong Province ; Special Fund for the Scholar Program of Yantai and Shandong Province ; Key Development Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences
收录类别	SCI
语种	英语
关键词[WOS]	ION-SELECTIVE ELECTRODES ; PULSED GALVANOSTATIC CONTROL ; MEMBRANE ELECTRODES ; CONDUCTING POLYMER ; RECOGNITION ; PULSTRODES ; COMPOSITE ; GRAPHENE ; CALCIUM ; ASSAY
研究领域[WOS]	Chemistry
WOS记录号	WOS:001235332900004
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/35993
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
通讯作者	Ding, Jiawang
作者单位	1.Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Jiarong,Ding, Jiawang,Luan, Feng,et al. Chronopotentiometric sensors for antimicrobial peptide-based biosensing of Staphylococcus aureus[J]. MICROCHIMICA ACTA,2024,191(6):11.
APA	Zhao, Jiarong,Ding, Jiawang,Luan, Feng,&Qin, Wei.(2024).Chronopotentiometric sensors for antimicrobial peptide-based biosensing of Staphylococcus aureus.MICROCHIMICA ACTA,191(6),11.
MLA	Zhao, Jiarong,et al."Chronopotentiometric sensors for antimicrobial peptide-based biosensing of Staphylococcus aureus".MICROCHIMICA ACTA 191.6(2024):11.