Insights into the discovery and intervention of metalloproteinase in marine hazardous jellyfish

doi:10.1016/j.jhazmat.2024.134526

	Insights into the discovery and intervention of metalloproteinase in marine hazardous jellyfish
	Yang, Fengling 1,2; Yang, Kai 1,2; Wang, Yi 1,2; Yao, Jinchi 2,3; Hua, Xiaoyu 2; Danso, Blessing 2; Wang, Yongfang 2; Liang, Hongyu 1,2; Wang, Mingke 4; Chen, Jingbo 2; Chen, Lingxin5 ; Xiao, Liang2 ; Zhang, Jing 1
发表期刊	JOURNAL OF HAZARDOUS MATERIALS
ISSN	0304-3894
	2024-07-05
卷号	472 页码:12
关键词	Chrysaora quinquecirrha Jellyfish Metalloproteinase Toxin Tetracycline hydrochloride
DOI	10.1016/j.jhazmat.2024.134526
通讯作者	Chen, Lingxin([email protected]) ; Xiao, Liang([email protected]) ; Zhang, Jing([email protected])
英文摘要	The proliferation of toxic organisms caused by changes in the marine environment, coupled with the rising human activities along the coastal lines, has resulted in an increasing number of stinging incidents, posing a serious threat to public health. Here, we evaluated the systemic toxicity of the venom in jellyfish Chrysaora quinquecirrha at both cellular and animal levels, and found that jellyfish tentacle extract (TE) has strong lethality accompanied by abnormal elevation of blood biochemical indicators and pathological changes. Joint analysis of transcriptome and proteome indicated that metalloproteinases are the predominant toxins in jellyfish. Specially, two key metalloproteinases DN6695_c0_g3 and DN8184_c0_g7 were identified by mass spectrometry of the red blood cell membrane and tetracycline hydrochloride (Tch) inhibition models. Structurally, molecular docking and kinetic analysis are employed and observed that Tch could inhibit the enzyme activity by binding to the hydrophobic pocket of the catalytic center. In this study, we demonstrated that Tch impedes the metalloproteinase activity thereby reducing the lethal effect of jellyfish, which suggests a potential strategy for combating the health threat of marine toxic jellyfish.
资助机构	National Key R & D Plan of the Ministry of Science and Technology Sino US International Cooperation Project ; National Natural Science Foundation of China
收录类别	SCI
语种	英语
关键词[WOS]	MATRIX ; PROTEINS ; FAMILY ; BLOOMS
研究领域[WOS]	Engineering ; Environmental Sciences & Ecology
WOS记录号	WOS:001237991000001
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/35960
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
通讯作者	Chen, Lingxin; Xiao, Liang; Zhang, Jing
作者单位	1.Jilin Agr Univ, Coll Tradit Chinese Med, Changchun 130118, Peoples R China 2.Naval Med Univ, Mil Med Univ 2, Fac Naval Med, Shanghai 200433, Peoples R China 3.Liaoning Normal Univ, Sch Life Sci, Dalian 116081, Peoples R China 4.Naval Med Univ, Med Care Ctr, Naval Med Ctr PLA, Shanghai 200052, Peoples R China 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Yang, Fengling,Yang, Kai,Wang, Yi,et al. Insights into the discovery and intervention of metalloproteinase in marine hazardous jellyfish[J]. JOURNAL OF HAZARDOUS MATERIALS,2024,472:12.
APA	Yang, Fengling.,Yang, Kai.,Wang, Yi.,Yao, Jinchi.,Hua, Xiaoyu.,...&Zhang, Jing.(2024).Insights into the discovery and intervention of metalloproteinase in marine hazardous jellyfish.JOURNAL OF HAZARDOUS MATERIALS,472,12.
MLA	Yang, Fengling,et al."Insights into the discovery and intervention of metalloproteinase in marine hazardous jellyfish".JOURNAL OF HAZARDOUS MATERIALS 472(2024):12.