Toxic effects of freshwater grouper (Acrossocheilus fasciatus) eggs on poultry: Morphological and transcriptomic insights into hepatic toxicity

doi:10.1016/j.ecoenv.2024.116684

	Toxic effects of freshwater grouper (Acrossocheilus fasciatus) eggs on poultry: Morphological and transcriptomic insights into hepatic toxicity
	Jiang, Yanqiu 1; Lu, Zhen 2; Wang, Derui 2,3; Hou, Zhihao 2; Zhong, Tianxing 1; Yan, Zhi 2,4; Lin, Ainuo 2; Jiang, Baozhen 2; Ren, Jianfeng 1; Li, Ke2
发表期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
	2024-08-01
卷号	281 页码:8
关键词	Acrossocheilus fasciatus Egg-toxin Hepatotoxicity Differentially expressed genes
DOI	10.1016/j.ecoenv.2024.116684
通讯作者	Ren, Jianfeng([email protected]) ; Li, Ke([email protected])
英文摘要	Fish egg poisoning is a serious and neglected public menace that kills hundreds of people and numerous poultry each year. Freshwater groupers (Acrossocheilus fasciatus) are common food fish in the southeastern regions of China. Their toxic eggs are regarded as a significant public health concern. The molecular mechanisms of eggtoxin toxicity in freshwater grouper to poisoned organisms are elusive. In this study, black-boned chicks were exposed to toxic eggs from freshwater grouper at a lethal dose. The hepatic morphology of the intoxicated chick was assessed. An analysis of the liver gene expression profile was conducted by comparing samples exposed to toxic eggs with control samples using RNA-Seq. The result revealed that an increase in vacuolation and congestion was observed in chicks with toxic eggs exposure. The transcriptome analysis revealed 5421 genes with differential expression, comprising 2810 up-regulated and 2611 down-regulated genes. The genes were primarily linked to energy metabolism, cell apoptosis, cell adhesion, exogenous microbial infection, and cell junction. The most strongly upregulated genes were cholecystokinin (CCK), cholecystokinin A receptor (CCKAR), and unc-80 homolog, NALCN activator (UNC80), and the most downregulated genes were glycine amidinotransferase (GATM), fatty acid desaturase 2 (FADS2), and hexokinase 2 (HKDC1). GO term with the highest enrichment of DEGs is nucleosome assembly. According to KEGG pathways, the three most significant metabolic pathways in the liver are DNA replication, retinol metabolism, and steroid biosynthesis. The results could be crucial for comprehending the negative biological impacts of egg-toxin and its toxic mechanisms. The outcome could provide potential biomarkers of egg-toxin exposure in hepatic, which might be useful for manufacturing an antidote to egg-toxin and providing valuable insights for ecotoxicity studies.
资助机构	National Natural Science Foundation of China ; National Key Research and Development Program of China ; Taishan Scholar Program from Shandong Province of China ; Yantai Municipal City's Shuangbai Plan
收录类别	SCI
语种	英语
关键词[WOS]	DEFENSE ; PREDATION
研究领域[WOS]	Environmental Sciences & Ecology ; Toxicology
WOS记录号	WOS:001265938100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/35850
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Ren, Jianfeng; Li, Ke
作者单位	1.Shanghai Ocean Univ, Key Lab Freshwater Aquat Genet Resources Certifica, Minist Agr & Rural Affairs, Shanghai 210306, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 3.Beibu Gulf Univ, Coll Marine Sci, Qinzhou 535011, Peoples R China 4.Yantai Univ, Sch Ocean, Yantai 264005, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Yanqiu,Lu, Zhen,Wang, Derui,et al. Toxic effects of freshwater grouper (Acrossocheilus fasciatus) eggs on poultry: Morphological and transcriptomic insights into hepatic toxicity[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2024,281:8.
APA	Jiang, Yanqiu.,Lu, Zhen.,Wang, Derui.,Hou, Zhihao.,Zhong, Tianxing.,...&Li, Ke.(2024).Toxic effects of freshwater grouper (Acrossocheilus fasciatus) eggs on poultry: Morphological and transcriptomic insights into hepatic toxicity.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,281,8.
MLA	Jiang, Yanqiu,et al."Toxic effects of freshwater grouper (Acrossocheilus fasciatus) eggs on poultry: Morphological and transcriptomic insights into hepatic toxicity".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 281(2024):8.