Exploring the innate immune system of Urechis unicinctus: Insights from full-length transcriptome analysis

doi:10.1016/j.gene.2024.148784

	Exploring the innate immune system of Urechis unicinctus: Insights from full-length transcriptome analysis
	Dong, Haomiao 1,2; Huang, Dong 1; Zhang, Jian 3; Xu, Dong 4; Jiao, Xudong1,2 ; Wang, Weizhong 4
发表期刊	GENE
ISSN	0378-1119
	2024-11-30
卷号	928 页码:16
关键词	Urechis unicinctus SMRT sequencing Full-length transcriptome Immune-related molecules Pattern recognition receptors
DOI	10.1016/j.gene.2024.148784
通讯作者	Jiao, Xudong([email protected]) ; Wang, Weizhong([email protected])
英文摘要	The Echiura worm Urechis unicinctus refers to a common benthic invertebrate found in the intertidal zone of Huanghai as well as Bohai Bay. U. unicinctus is known to contain various physiologically active substances, making it highly valuable in terms of its edibility, medicinal properties, and economic potential. Nonetheless, the limited study on the immune system of U. unicinctus poses difficulties for its aquaculture and artificial reproduction. Marine invertebrates, including shellfish and U. unicinctus, , are thought to primarily depend on their innate immune system for disease protection, owing to the several innate immune molecules they possess. Herein, we employed PacBio single-molecule real-time (SMRT) sequencing technology to perform the full-length transcriptome analysis of U. unicinctus individuals under five different conditions (room temperature (RT), low temperature (LT), high temperature (HT), without water (DRY), ultraviolet irradiation (UV)). Concequently, we identified 59,371 unigenes that had a 2,779 bp average length, 2,613 long non-coding RNAs (lncRNAs), 59,190 coding sequences (CDSs), 35,166 simple sequence repeats (SSRs), and 1,733 transcription factors (TFs), successfully annotating 90.58% (53,778) of the unigenes. Subsequently, key factors associated with immune-related processes, such as non-self-recognition, cellular immune defenses, and humoral immune defenses, were searched. Our study also identified pattern recognition receptors (PRRs) that included 17 peptidoglycan recognition proteins (PGRPs), 13 Gram-negative binding proteins (GNBPs), 18 scavenger receptors (SRs), 74 toll-like receptors (TLRs), and 89 C-type lectins (CLTs). Altogether, the high-quality transcriptome obtained data will offer valuable insights for further investigations into U. unicinctus innate immune response, laying the foundation for subsequent molecular biology studies and aquaculture.
资助机构	Cultivation Project for Leading Enterprises in Aquatic Seed Industry of Shandong and Improvement of Aquatic Seed Industry Project of Yantai
收录类别	SCI
语种	英语
关键词[WOS]	GRAM-NEGATIVE BACTERIA ; LONG NONCODING RNAS ; PATHOGEN RECOGNITION ; LOW-TEMPERATURE ; RECEPTOR ; PROTEIN ; GENES ; EXPRESSION ; IDENTIFICATION ; INDUCTION
研究领域[WOS]	Genetics & Heredity
WOS记录号	WOS:001289567400001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/35832
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Jiao, Xudong; Wang, Weizhong
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Yantai Univ, Sch Ocean, Yantai 264005, Peoples R China 4.Shandong Blue Ocean Technol Co Ltd, Yantai 261400, Peoples R China
推荐引用方式 GB/T 7714	Dong, Haomiao,Huang, Dong,Zhang, Jian,et al. Exploring the innate immune system of Urechis unicinctus: Insights from full-length transcriptome analysis[J]. GENE,2024,928:16.
APA	Dong, Haomiao,Huang, Dong,Zhang, Jian,Xu, Dong,Jiao, Xudong,&Wang, Weizhong.(2024).Exploring the innate immune system of Urechis unicinctus: Insights from full-length transcriptome analysis.GENE,928,16.
MLA	Dong, Haomiao,et al."Exploring the innate immune system of Urechis unicinctus: Insights from full-length transcriptome analysis".GENE 928(2024):16.