Peptide-Driven Assembly of Magnetic Beads for Potentiometric Sensing of Bacterial Enzyme at a Subcellular Level

doi:10.1021/acssensors.4c01632

	Peptide-Driven Assembly of Magnetic Beads for Potentiometric Sensing of Bacterial Enzyme at a Subcellular Level
	Zhang, Han 1,4; Mou, Junsong 1,4; Ding, Jiawang1,4 ; Qin, Wei1,2,3,4
发表期刊	ACS SENSORS
ISSN	2379-3694
	2024-08-24
页码	9
关键词	potentiometric sensing subcellular enzyme peptide assembly bacteria alkaline phosphatase
DOI	10.1021/acssensors.4c01632
通讯作者	Ding, Jiawang([email protected])
英文摘要	Bacterial enzymes with different subcellular localizations play a critical ecological role in biogeochemical processing. However, precisely quantifying enzymes localized at certain subcellular levels, such as extracellular enzymes, has not yet been fully realized due to the complexity and dynamism of the bacterial outer membrane. Here we present a magneto-controlled potentiometric sensing platform for the specific detection of extracellular enzymatic activity. Alkaline phosphatase (ALP), which is one of the crucial hydrolytic enzymes in the ocean, was selected as the target enzyme. Magnetic beads functionalized with an ALP-responsive self-assembled peptide (GGGGGFFFpYpYEEE, MBs-peptides) prevent negatively charged peptides from entering the bacterial outer membrane, thereby enabling direct potentiometric sensing of extracellular ALP both attached to the bacterial cell surface and released into the surrounding environment. The dephosphorylation-triggered assembly of peptide-coupled magnetic beads can be directly and sensitively measured by using a magneto-controlled sensor. In this study, extracellular ALP activity of Pseudomonas aeruginosa at concentrations ranging from 10 to 1.0 x 10(5) CFU mL(-1) was specifically and sensitively monitored. Moreover, this magneto-controlled potentiometric method enabled a simple and accurate assay of ALP activity across different subcellular localizations.
资助机构	National Natural Science Foundation of China ; Taishan Scholar Program of Shandong Province ; Special Fund for the Scholar Program of Yantai and Shandong Province ; Key Development Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences ; Seed Project of Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences
收录类别	SCI
语种	英语
关键词[WOS]	ALKALINE-PHOSPHATASE ACTIVITY ; PHOSPHORUS ; COMPONENT
研究领域[WOS]	Chemistry ; Science & Technology - Other Topics
WOS记录号	WOS:001298226700001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/35777
专题	中国科学院海岸带环境过程与生态修复重点实验室中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
通讯作者	Ding, Jiawang
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Han,Mou, Junsong,Ding, Jiawang,et al. Peptide-Driven Assembly of Magnetic Beads for Potentiometric Sensing of Bacterial Enzyme at a Subcellular Level[J]. ACS SENSORS,2024:9.
APA	Zhang, Han,Mou, Junsong,Ding, Jiawang,&Qin, Wei.(2024).Peptide-Driven Assembly of Magnetic Beads for Potentiometric Sensing of Bacterial Enzyme at a Subcellular Level.ACS SENSORS,9.
MLA	Zhang, Han,et al."Peptide-Driven Assembly of Magnetic Beads for Potentiometric Sensing of Bacterial Enzyme at a Subcellular Level".ACS SENSORS (2024):9.