High-yield magnetosome production of <i>Magnetospirillum magneticum</i> strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation

doi:10.1007/s10529-024-03507-x

	*High-yield magnetosome production of Magnetospirillum magneticum* strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation**
	Wang, Yu 1,2; Liu, Zhengyi1,2 ; Li, Wenjun1,2 ; Cui, Hongli1,2 ; Huang, Yandi 3; Qin, Song1,2
发表期刊	BIOTECHNOLOGY LETTERS
ISSN	0141-5492
	2024-07-20
页码	15
关键词	Flask fermentation High yield Magnetosome Magnetotactic bacterium AMB-1 Pathway optimization Reductase activity
DOI	10.1007/s10529-024-03507-x
通讯作者	Qin, Song([email protected])
英文摘要	Objectives Developing a simplified flask fermentation strategy utilizing magnetotactic bacterium AMB-1 and optimized iron supplementation for high-yield magnetosome production to address the challenges associated with magnetosome acquisition. Results A reliable processing for the pure culture of AMB-1 was established using standard laboratory consumables and equipment. Subsequently, the medium and iron supplementation were optimized to enhance the yield of AMB-1 magnetosomes. The mSLM supported higher biomass accumulation in flask fermentation, reaching an OD565 of similar to 0.7. The premixed solution of ferric quinate and EDTA-Fe (at a ratio of 0.5:0.5 and a concentration of 0.4 mmol/L) stabilized Fe3+ and significantly increased the reductase activity of AMB-1. Flask fermentations with an initial volume of 15 L were then conducted employing the optimized fermentation strategy. After two rounds of iron and nutrient supplementation, the magnetosome yield reached 185.7 +/- 9.5 mg/batch (approximately 12 mg/L), representing the highest AMB-1 flask fermentation yield to our knowledge. Conclusion A flask fermentation strategy for high-yield magnetsome production was developed, eliminating the need for bioreactors and greatly simplifying the process of magnetosome acquisition.
资助机构	Department of Science and Technology of Shandong Province
收录类别	SCI
语种	英语
关键词[WOS]	MAGNETOTACTIC BACTERIA ; NANOPARTICLES ; GROWTH
研究领域[WOS]	Biotechnology & Applied Microbiology
WOS记录号	WOS:001273147500001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/35709
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Qin, Song
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, 17,Chunhui Rd, Yantai 264003, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Yantai Univ, Sch Life Sci, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yu,Liu, Zhengyi,Li, Wenjun,et al. High-yield magnetosome production of Magnetospirillum magneticum strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation[J]. BIOTECHNOLOGY LETTERS,2024:15.
APA	Wang, Yu,Liu, Zhengyi,Li, Wenjun,Cui, Hongli,Huang, Yandi,&Qin, Song.(2024).High-yield magnetosome production of Magnetospirillum magneticum strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation.BIOTECHNOLOGY LETTERS,15.
MLA	Wang, Yu,et al."High-yield magnetosome production of Magnetospirillum magneticum strain AMB-1 in flask fermentation through simplified processing and optimized iron supplementation".BIOTECHNOLOGY LETTERS (2024):15.