PoCXCL8, a teleost chemokine, exerts direct bactericidal, chemotactic/ phagocytic, and NETs releasing properties, promoting host anti-bacterial immunity

doi:10.1016/j.fsi.2024.109874

	PoCXCL8, a teleost chemokine, exerts direct bactericidal, chemotactic/ phagocytic, and NETs releasing properties, promoting host anti-bacterial immunity
	Zhao, Kun-yu 1; Chen, Guan-yu 1; Huang, Hui 2; Jiao, Xu-dong3 ; Li, Xue-peng 1; Zhang, Jian 1
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2024-11-01
卷号	154 页码:12
关键词	Paralichthys olivaceus CXCL8 Antibacterial immune Leukocyte vitality
DOI	10.1016/j.fsi.2024.109874
通讯作者	Li, Xue-peng([email protected]) ; Zhang, Jian([email protected])
英文摘要	As an important CXC chemokine, CXCL8 plays pleiotropic roles in immunological response. In teleost, CXCL8 is involved in cell migration and bacterial invasion. However, the immune antibacterial function of CXCL8 in Japanese flounder (Paralichthys olivaceus) (PoCXCL8) is largely scarce. In this research, we investigated the antibacterial property and leukocyte activation of PoCXCL8. PoCXCL8 consists of 100 amino acid residues, with a conserved chemokine CXC domain. PoCXCL8 was expressed in various tissues, with the highest level in liver and the lowest level in muscle, and sharply induced by V. harveyi or E. tarda in liver, spleen, and head kidney. In vitro, the recombinant PoCXCL8 (rPoCXCL8) could bind to Bacillus subtilis, Edwardsiella tarda, Escherichia coli, Pseudomonas fluorescens, Vibrio anguillarum, Vibrio harveyi, Staphylococcus aureus, and Micrococcus luteus, affect the growth of E. coli, E. tarda, M. luteus, and P. fluorescens, and have a direct bactericidal effect on E. coli and E. tarda. Moreover, rPoCXCL8 was able to bind the outer membranal protein rPilA of E. tarda. In addition, rPoCXCL8 could bind to PBLs, activating the PBLs activity including chemotaxis, proliferation, phagocytosis, reactive oxygen species, acid phosphatase activity. At same time, rPoCXCL8 could induce neutrophil to generate neutrophil extracellular traps (NETs) and promote the expression of inflammatory genes including IL-1 beta, IL6, MMP13, TNF alpha, and NF-kappa B. In flounder, the presence of rPoCXCL8 could enhance the in vivo resistance to E. tarda in liver, spleen, and head kidney. Moreover, the PoCXCL8-deficient could attenuate the fish defense against E. tarda infection in in spleen and head kidney. In conclusion, these results provided new insights into the antibacterial properties of CXCL8 in P. olivaceus.
资助机构	Development Plan of Youth Innovation Team in Colleges and Universities of Shandong Province ; Yantai Key Laboratory of Quality and Safety Control and Deep Processing of Marine Food ; Yantai University Doctoral Start-up Foundation
收录类别	SCI
语种	英语
关键词[WOS]	MOLECULAR CHARACTERIZATION ; CYNOGLOSSUS-SEMILAEVIS ; EXPRESSION ANALYSIS ; EDWARDSIELLA-TARDA ; 1ST CHARACTERIZATION ; EXTRACELLULAR TRAPS ; INTERLEUKIN-8 ; CYTOKINES ; CLONING ; IL-8
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:001313519600001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/35636
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Li, Xue-peng; Zhang, Jian
作者单位	1.Yantai Univ, Sch Ocean, Yantai, Peoples R China 2.Shandong Marine Resource & Environm Res Inst, Yantai, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Kun-yu,Chen, Guan-yu,Huang, Hui,et al. PoCXCL8, a teleost chemokine, exerts direct bactericidal, chemotactic/ phagocytic, and NETs releasing properties, promoting host anti-bacterial immunity[J]. FISH & SHELLFISH IMMUNOLOGY,2024,154:12.
APA	Zhao, Kun-yu,Chen, Guan-yu,Huang, Hui,Jiao, Xu-dong,Li, Xue-peng,&Zhang, Jian.(2024).PoCXCL8, a teleost chemokine, exerts direct bactericidal, chemotactic/ phagocytic, and NETs releasing properties, promoting host anti-bacterial immunity.FISH & SHELLFISH IMMUNOLOGY,154,12.
MLA	Zhao, Kun-yu,et al."PoCXCL8, a teleost chemokine, exerts direct bactericidal, chemotactic/ phagocytic, and NETs releasing properties, promoting host anti-bacterial immunity".FISH & SHELLFISH IMMUNOLOGY 154(2024):12.