| VEGF gene silencing by cytomegalovirus promoter driven ShRNA expression vector results in vascular development defects in zebrafish | |
Yang, S. L.1 ; Yan, S.3; Niu, R. L.2; Lin, X. K.2
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| 发表期刊 | RUSSIAN JOURNAL OF GENETICS
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| ISSN | 1022-7954 |
| 2009-09-01 | |
| 卷号 | 45期号:9页码:1040-1046 |
| 关键词 | Endothelial Growth-factor Pol-ii Promoter Rna Interference Angiogenesis Hematopoiesis Neuropilin-1 Mammals System Cell |
| 产权排序 | CAS, Yantai Inst Coastal Zone Res Sustainable Dev;CAS, Inst Oceanol, Key Lab Marine Biol; Dalian Jiaotong Univ, Coll Environm & Chem Engn |
| 通讯作者 | Yang, SL, CAS, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China |
| 作者部门 | 生物资源实验室 |
| 英文摘要 | Zebrafish has been generally considered as an excellent model in case of drug screening, disease model establishment, and vertebrate embryonic development study. In this work, the ability of human cytomegalovirus immediate early promoter (CMV promoter)-driven short hairpin RNA (shRNA) expression vector to induce shRNA against VEGF gene in zebrafish was tested, and its effect on vascular development was assed, too. Using RT-qPCR, blood vessel staining, and in situ hybridization, we confirmed certain transcriptional activity and down regulation of gene expression by the vector. In situ hybridization analysis indicated selective inhibition of NRP1 expression in the VEGF gene loss of function model, which might imply in turn that VEGF could not only activate endothelial cells directly but also could contribute to stimulating angiogenesis in vivo by a mechanism that involved up-regulation of its cognate receptor expression in zebrafish. This contributed to a better understanding of molecular mechanisms of cardiovascular development. The system improved the success rate in making inducible knockdown and widened the possibilities for better therapeutic targets in zebrafish. |
| 文章类型 | Article |
| 资助机构 | key technology research and development programme of Shandong Province [2007GG2QT06020]; National Natural Science Foundation of China [40506031] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | ENDOTHELIAL GROWTH-FACTOR ; POL-II PROMOTER ; RNA INTERFERENCE ; ANGIOGENESIS ; HEMATOPOIESIS ; NEUROPILIN-1 ; MAMMALS ; SYSTEM ; CELL |
| 研究领域[WOS] | Genetics & Heredity |
| WOS记录号 | WOS:000269974100003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/3485 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.CAS, Yantai Inst Coastal Zone Res Sustainable Dev, Yantai 264003, Peoples R China 2.CAS, Inst Oceanol, Key Lab Marine Biol, Qingdao 266071, Peoples R China 3.Dalian Jiaotong Univ, Coll Environm & Chem Engn, Dalian 116028, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, S. L.,Yan, S.,Niu, R. L.,et al. VEGF gene silencing by cytomegalovirus promoter driven ShRNA expression vector results in vascular development defects in zebrafish[J]. RUSSIAN JOURNAL OF GENETICS,2009,45(9):1040-1046. |
| APA | Yang, S. L.,Yan, S.,Niu, R. L.,&Lin, X. K..(2009).VEGF gene silencing by cytomegalovirus promoter driven ShRNA expression vector results in vascular development defects in zebrafish.RUSSIAN JOURNAL OF GENETICS,45(9),1040-1046. |
| MLA | Yang, S. L.,et al."VEGF gene silencing by cytomegalovirus promoter driven ShRNA expression vector results in vascular development defects in zebrafish".RUSSIAN JOURNAL OF GENETICS 45.9(2009):1040-1046. |
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