Proteomic analysis of the regulatory networks of ClpX in a model cyanobacterium Synechocystis sp. PCC 6803

doi:10.3389/fpls.2022.994056

YIC-IR

	Proteomic analysis of the regulatory networks of ClpX in a model cyanobacterium Synechocystis sp. PCC 6803
	Zhang, Yumeng 1,2,3; Wang, Yaqi 1,2,3; Wei, Wei 1,2,3; Wang, Min 4; Jia, Shuzhao 4; Yang, Mingkun 1,2,3; Ge, Feng 1,2,3
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
	2022-09-29
卷号	13 页码:16
关键词	cyanobacteria proteases proteostasis quantitative proteomics ClpX
DOI	10.3389/fpls.2022.994056
通讯作者	Yang, Mingkun([email protected]) ; Ge, Feng([email protected])
英文摘要	Protein homeostasis is tightly regulated by protein quality control systems such as chaperones and proteases. In cyanobacteria, the ClpXP proteolytic complex is regarded as a representative proteolytic system and consists of a hexameric ATPase ClpX and a tetradecameric peptidase ClpP. However, the functions and molecular mechanisms of ClpX in cyanobacteria remain unclear. This study aimed to decipher the unique contributions and regulatory networks of ClpX in the model cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis). We showed that the interruption of clpX led to slower growth, decreased high light tolerance, and impaired photosynthetic cyclic electron transfer. A quantitative proteomic strategy was employed to globally identify ClpX-regulated proteins in Synechocystis cells. In total, we identified 172 differentially expressed proteins (DEPs) upon the interruption of clpX. Functional analysis revealed that these DEPs are involved in diverse biological processes, including glycolysis, nitrogen assimilation, photosynthetic electron transport, ATP-binding cassette (ABC) transporters, and two-component signal transduction. The expression of 24 DEPs was confirmed by parallel reaction monitoring (PRM) analysis. In particular, many hypothetical or unknown proteins were found to be regulated by ClpX, providing new candidates for future functional studies on ClpX. Together, our study provides a comprehensive ClpX-regulated protein network, and the results serve as an important resource for understanding protein quality control systems in cyanobacteria.
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; China Postdoctoral Science Foundation ; State Key Laboratory of Freshwater Ecology and Biotechnology
收录类别	SCI
语种	英语
关键词[WOS]	SP PCC-6803 ; PROTEASE ; SYSTEM ; DEGRADATION ; PROTEOLYSIS ; RESISTANCE ; REVEALS ; STRESS ; IDENTIFICATION ; POLYPHOSPHATE
研究领域[WOS]	Plant Sciences
WOS记录号	WOS:000868254800001
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/34809
专题	中国科学院烟台海岸带研究所
通讯作者	Yang, Mingkun; Ge, Feng
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China 3.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Anal & Testing Ctr, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yumeng,Wang, Yaqi,Wei, Wei,et al. Proteomic analysis of the regulatory networks of ClpX in a model cyanobacterium Synechocystis sp. PCC 6803[J]. FRONTIERS IN PLANT SCIENCE,2022,13:16.
APA	Zhang, Yumeng.,Wang, Yaqi.,Wei, Wei.,Wang, Min.,Jia, Shuzhao.,...&Ge, Feng.(2022).Proteomic analysis of the regulatory networks of ClpX in a model cyanobacterium Synechocystis sp. PCC 6803.FRONTIERS IN PLANT SCIENCE,13,16.
MLA	Zhang, Yumeng,et al."Proteomic analysis of the regulatory networks of ClpX in a model cyanobacterium Synechocystis sp. PCC 6803".FRONTIERS IN PLANT SCIENCE 13(2022):16.