| Successively amplified electrochemical immunoassay based on biocatalytic deposition of silver nanoparticles and silver enhancement | |
Chen, Zhao-Peng ; Peng, Zhao-Feng; Luo, Yan; Qu, Bo; Jiang, Jian-Hui; Zhang, Xiao-Bing; Shen, Guo-Li; Yu, Ru-Qin
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| 发表期刊 | BIOSENSORS & BIOELECTRONICS
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| 2007-11-30 | |
| 卷号 | 23期号:4页码:485-491 |
| 关键词 | Electrochemical Immunoassay Biocatalytic Deposition Silver Nanoparticles Silver Enhancement Anodic Stripping Voltammetry |
| 产权排序 | China Earthquake Adm, Inst Earthquake Sci; Chinese Acad Sci, Lanzhou Inst Geol; Chinese Acad Sci, Inst Geochem, Guiyang 550002, Peoples R China; Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev |
| 通讯作者 | Yu, RQ |
| 作者部门 | 环境化学实验室 |
| 英文摘要 | A successively signal-amplified electrochemical immunoassay has been reported on the basis of the biocatalytic deposition of silver nanoparticles with their subsequent enlargement by nanoparticle-promoted catalytic precipitation of silver from the silver-enhancer solution. The immunoassay was carried out based on a heterogeneous sandwich procedure using polystyrene microwells to immobilize antibody. After all the processes comprising the formation of immunocomplex, biocatalytic deposition of silver nanoparticles and following silver enhancement were completed, the silver on polystyrene microwells was dissolved and quantified by anodic stripping voltammetry (ASV). The effect of relevant experimental conditions, including the concentration of ascorbic acid 2-phosphate (AA-p) substrate and Ag(I) ions, the biocatalytic deposition time, and of crucial importance, the silver enhancement time, were investigated and optimized. The anodic stripping peak current was proportional to the concentration of human IgG in a dynamic range of 0.1-10 ng ml(-1) with a detection limit of 0.03 ng ml(-1). Scanning electron microscope (SEM) was applied to characterize the silver nanoparticles before and after silver enhancement on the surface of polystyrene microplates. By coupling the highly catalytic effect of enzyme and nanoparticles to successively amplify the analytical signal, the sensitivity of immunoassay was enhanced so dramatically that this approach would be a promising strategy to achieve a lower detection limit for bioassays. (c) 2007 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | FARADAIC IMPEDANCE SPECTROSCOPY ; SURFACE-PLASMON RESONANCE ; DNA HYBRIDIZATION ; STRIPPING VOLTAMMETRY ; ELECTRON-MICROSCOPE ; SIGNAL-TRANSDUCTION ; GOLD ; SENSORS ; LABEL ; CHRONOPOTENTIOMETRY |
| 研究领域[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS记录号 | WOS:000251120200006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/3457 |
| 专题 | 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心 中国科学院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室 |
| 作者单位 | 1.Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemomet, Changsha 410082, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res Sustainable Dev, Dept Environm Chem, Yantai 264003, Peoples R China 3.Hunan Normal Univ, Chem & Chem Engn Coll, Key Lab Biol & Tradit Chinese Med Res, Minist Educ, Changsha 410081, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Zhao-Peng,Peng, Zhao-Feng,Luo, Yan,et al. Successively amplified electrochemical immunoassay based on biocatalytic deposition of silver nanoparticles and silver enhancement[J]. BIOSENSORS & BIOELECTRONICS,2007,23(4):485-491. |
| APA | Chen, Zhao-Peng.,Peng, Zhao-Feng.,Luo, Yan.,Qu, Bo.,Jiang, Jian-Hui.,...&Yu, Ru-Qin.(2007).Successively amplified electrochemical immunoassay based on biocatalytic deposition of silver nanoparticles and silver enhancement.BIOSENSORS & BIOELECTRONICS,23(4),485-491. |
| MLA | Chen, Zhao-Peng,et al."Successively amplified electrochemical immunoassay based on biocatalytic deposition of silver nanoparticles and silver enhancement".BIOSENSORS & BIOELECTRONICS 23.4(2007):485-491. |
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