hUMSCs regulate the differentiation of ovarian stromal cells via TGF-β 1

YIC-IR

	hUMSCs regulate the differentiation of ovarian stromal cells via TGF-β 1
其他题名	hUMSCs regulate the differentiation of ovarian stromal cells via TGF-β 1
	Linlu Cui 1; Hongchu Bao 2; Zhongfeng Liu 2; Xuejing Man 2; Hongyuan Liu 1; Yun Hou 1; Qianqian Luo 1; Siyuan Wang 1; Qiang Fu 1; Hongqin Zhang 1
发表期刊	Stem Cell Research & Therapy
ISSN	1757-6512
	2020
卷号	11 期号:1
英文摘要	Objective The basic pathological changes of primary ovarian insufficiency (POI) include ovarian tissue fibrosis and follicular development disorders. The human umbilical cord mesenchymal stem cell (hUMSC) transplantation has been shown an effective method to improve the ovarian function in POI rat model; however, the exact mechanisms are still unclear. The purpose of this study is to investigate whether the recovery of ovarian function in POI rats is related to the inhibition of tissue fibrosis following hUMSC transplantation. Furthermore, the transforming growth factor-β 1 (TGF-β 1 ) signaling pathway is explored to determine the mechanisms of ovarian function recovery through its inhibition of tissue fibrosis. Methods The primary ovarian insufficiency (POI) rat model was established by intraperitoneal injection of chemotherapy drug cisplatin (CDDP) for 7?days. The levels of serum sex hormones were measured using enzyme-linked immunosorbent assay (ELISA). The tissue fibrosis in the ovary was examined using Masson staining and Sirius red staining. The collagen fibers in the ovarian tissues were detected by Western blot analysis. To investigate the mechanisms of ovarian function recovery following hUMSC transplantation, ovarian stromal cells were isolated from the ovarian cortex of immature rats. The expression of Cytochrome P450 17A1 (Cyp17a1) and fibrosis marker of alpha smooth muscle actin (α-SMA) in ovarian stromal cells was examined using immunofluorescence analysis. Also, the protein levels of Cyp17a1 and α-SMA in ovarian stromal cells were examined by Western blot analysis. The expression of TGF-β 1 and Smad3 signals was measured by Western blot and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis. Results The results show that the function of the ovary in POI rats was significantly improved after hUMSC transplantation. The expression of fibrosis markers (α-SMA) and production of Collagen Type I (Collagen I) and Collagen Type III (Collagen III) in POI rats were significantly inhibited in POI rats following hUMSC transplantation. In the cultured ovarian stromal cells, the decrease of TGF-β 1 and p-Smad3 protein expression was observed in hUMSC-treated POI rats. The treatment with TGF-β 1 inhibitor of SB431542 further confirmed this signal pathway was involved in the process. Conclusion Our study demonstrated that the TGF-β 1
中文摘要	Objective The basic pathological changes of primary ovarian insufficiency (POI) include ovarian tissue fibrosis and follicular development disorders. The human umbilical cord mesenchymal stem cell (hUMSC) transplantation has been shown an effective method to improve the ovarian function in POI rat model; however, the exact mechanisms are still unclear. The purpose of this study is to investigate whether the recovery of ovarian function in POI rats is related to the inhibition of tissue fibrosis following hUMSC transplantation. Furthermore, the transforming growth factor-β 1 (TGF-β 1 ) signaling pathway is explored to determine the mechanisms of ovarian function recovery through its inhibition of tissue fibrosis. Methods The primary ovarian insufficiency (POI) rat model was established by intraperitoneal injection of chemotherapy drug cisplatin (CDDP) for 7?days. The levels of serum sex hormones were measured using enzyme-linked immunosorbent assay (ELISA). The tissue fibrosis in the ovary was examined using Masson staining and Sirius red staining. The collagen fibers in the ovarian tissues were detected by Western blot analysis. To investigate the mechanisms of ovarian function recovery following hUMSC transplantation, ovarian stromal cells were isolated from the ovarian cortex of immature rats. The expression of Cytochrome P450 17A1 (Cyp17a1) and fibrosis marker of alpha smooth muscle actin (α-SMA) in ovarian stromal cells was examined using immunofluorescence analysis. Also, the protein levels of Cyp17a1 and α-SMA in ovarian stromal cells were examined by Western blot analysis. The expression of TGF-β 1 and Smad3 signals was measured by Western blot and quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis. Results The results show that the function of the ovary in POI rats was significantly improved after hUMSC transplantation. The expression of fibrosis markers (α-SMA) and production of Collagen Type I (Collagen I) and Collagen Type III (Collagen III) in POI rats were significantly inhibited in POI rats following hUMSC transplantation. In the cultured ovarian stromal cells, the decrease of TGF-β 1 and p-Smad3 protein expression was observed in hUMSC-treated POI rats. The treatment with TGF-β 1 inhibitor of SB431542 further confirmed this signal pathway was involved in the process. Conclusion Our study demonstrated that the TGF-β 1
语种	英语
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/34527
专题	中国科学院烟台海岸带研究所
作者单位	1.滨州医学院 2.Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences
推荐引用方式 GB/T 7714	Linlu Cui,Hongchu Bao,Zhongfeng Liu,et al. hUMSCs regulate the differentiation of ovarian stromal cells via TGF-β 1[J]. Stem Cell Research & Therapy,2020,11(1).
APA	Linlu Cui.,Hongchu Bao.,Zhongfeng Liu.,Xuejing Man.,Hongyuan Liu.,...&Hongqin Zhang.(2020).hUMSCs regulate the differentiation of ovarian stromal cells via TGF-β 1.Stem Cell Research & Therapy,11(1).
MLA	Linlu Cui,et al."hUMSCs regulate the differentiation of ovarian stromal cells via TGF-β 1".Stem Cell Research & Therapy 11.1(2020).