4种不同涂膜处理对软枣猕猴桃贮藏品质的影响

	4种不同涂膜处理对软枣猕猴桃贮藏品质的影响
其他题名	The degradation of DDTs by Burkholderia xenovorans LB400 biphenyl dioxygenase and mutants
	王乐兵 1,2; 彭承祥 3; 孙晨晨 3; 孙林 2; 钟志海 2; 李德海 1; 刘正一2
发表期刊	食品安全质量检测学报
ISSN	2095-0381
	2023
卷号	14 期号:9 页码:232-239
关键词	软枣猕猴桃水果保鲜寡糖多糖酶活力
英文摘要	Dicholodiphenyltrichloroethanes (DDTs)is probably the best known and typical persistent organic pollutant in the world, which has been widely used in malaria control and agricultural deworming.They are still detected in various environmental matrices and has new input sources although their usage in agriculture has been banned in China and others.Numerous concerns have arisen over the past decades about the adverse environmental impacts(including harm to offshore ecosystem and human health)of DDTs.There has been a considerable interest over the last decades for the Rieske-type arylhydroxylating dioxygenases (RHDs)as they are seen as potentially capable of initiating their degradation.[Objective] In order to explore the degradation characteristics and mechanism of biphenyl dioxygenase(BPDO)on DDTs, we selected Burkholderia xenovorans LB400 biphenyl dioxygenase and its mutants to explore the degradation process of p,p'-DDT and o,p'-DDT.[Methods] Using BphAE_(LB400) as parent, the mutant BphAE_(S283M) was obtained by two-step site-directed mutagenesis from Ser to Met.The degradation characteristics and mechanism of wild type and mutant were explored by comparing the catalytic performance of wild type and mutant to DDTs, simulating the structure of mutant protein and molecular docking.[Results] The data showed that BphAE_(LB400) and BphAE_(S283M) could not be degraded p,p'-DDT, but BphAE_(S283M) metabolized o,p'-DDT and produced two stereoisomers.The structural analysis of BphAE_(LB400) and BphAE_(S283M) showed that the reaction ring of p,p'-DDT did not coincide with the biphenyl reaction ring in the original crystal structure.In the o,p'-DDT-BphAE_(S283M) conformation, the proximal ring did not fit the biphenyl reactive ring as well, but its orientation toward the catalytic Fe~(2+) places two vicinal atoms at a distance(within 0.5 nm)that would allow a catalytic reaction.In addition, the surface area and volume of the catalytic cavity of BphAE_(S283M) is larger than that of BphAE_(LB400), which is likely to contribute to the combination of BphAE_(S283M) and o,p'-DDT.[Conclusion] 283 is the key amino acid residue that affects the catalytic metabolism of BPDO to DDTs.It can affect the substrate specificity by adjusting the distance between the reaction carbon atom and the catalytic center and the size of the catalytic cavity.This will provide better insights about the bases for BPDO broad substrate range and about the mechanisms by which the enzyme evolves to change or expand its substrate range and its stereo-and regiospecificity.
中文摘要	目的探究不同涂膜处理对采后软枣猕猴桃贮藏品质的影响。方法以烟台“丰绿”软枣猕猴桃为实验材料,用褐藻酸钠、褐藻寡糖、壳聚糖、壳寡糖4种涂膜剂对采摘后的软枣猕猴桃进行涂膜处理,对其贮藏期间相关品质指标及果实形态变化进行检测分析。结果在20℃贮藏过程中,与对照组相比,4种处理在一定时间内均能抑制软枣猕猴桃果实质量损失率和软化程度,减少可溶性固形物、维生素C含量的下降,有效控制果实腐烂率。在贮藏过程中可使软枣猕猴桃果实保持较高水平的超氧化物歧化酶、过氧化氢酶、过氧化物酶活力。结论综合分析,成熟的软枣猕猴桃在20℃贮藏环境中,褐藻寡糖涂膜处理保鲜效果最优,更有利于果实的贮藏。
文章类型	期刊论文
收录类别	CSCD
语种	中文
CSCD记录号	CSCD:7086411
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/34223
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位	1.东北林业大学林学院,哈尔滨150040; 2.中国科学院烟台海岸带研究所,烟台264003; 3.大连海洋大学海洋科技与环境学院,大连116023
推荐引用方式 GB/T 7714	王乐兵,彭承祥,孙晨晨,等. 4种不同涂膜处理对软枣猕猴桃贮藏品质的影响[J]. 食品安全质量检测学报,2023,14(9):232-239.
APA	王乐兵.,彭承祥.,孙晨晨.,孙林.,钟志海.,...&刘正一.(2023).4种不同涂膜处理对软枣猕猴桃贮藏品质的影响.食品安全质量检测学报,14(9),232-239.
MLA	王乐兵,et al."4种不同涂膜处理对软枣猕猴桃贮藏品质的影响".食品安全质量检测学报 14.9(2023):232-239.