The invention relates to a sensor, in particular to a polymer liquid film potential sensor for detecting enzymatic activity and a detection method thereof. A potential detector is respectively connected with an internal reference electrode and an external reference electrode through wires, the internal reference electrode is inserted in a working electrode filled with internal liquid, the working electrode and the external reference electrode are inserted in a detection pool with detection liquid, the working electrode is an electrode carrier on which a polymer sensitive film is fixed, and a power device with a solution disturbance function is arranged in the detection pool. According the detection method, the potential detector is used for detecting the change of the potential of the polymer sensitive film at the bottom of the working electrode before and after enzymatic reaction, and the enzymatic activity concentration can be detected on the basis of a standard working curve according to a potential change signal. The potential sensor has the advantages of high generality, good selectivity, high sensitivity and low cost and is suitable for detection under a complicated matrix condition.
; 王学伟; 丁家旺
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