The invention discloses a detection system and a detection method of petroleum-type pollutants. The detection system comprises an emitting unit, a receiving unit, a reference unit and a signal processing unit. The emitting unit is used for emitting laser signals, and transmitted light signals and reflected light signals are outputted; an input terminal of the reference unit receives the transmitted light signals emitted from the emitting unit. An output terminal of the reference unit is connected to an input terminal of the signal processing unit. Reference signals outputted by the reference unit are transmitted to the signal receiving unit by the output terminal of the reference unit. A sample in a sample tank is excited by the reflected light signals, such that fluorescence signals are generated. The fluorescence signals are received by the receiving unit beside the sample tank, and are outputted to the signal processing unit by the receiving unit. The fluorescence signals are processed by the signal processing unit. The system and the method provided by the invention are based on laser-induced fluorescence detection principles. Emitted signals are modulated by using a modulation technology, such that the detection sensitivity of the system is high. With the method and the system, rapid, real-time, on-line and non-contact measuring upon water body petroleum-type pollutant fluorescence signals can be realized. The system and the method can be widely applied in water body environment monitoring.
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