The invention relates to fluorescent probe molecular detection, in particular to naphthalimide-modified rhodamine derivative (naphthalimide modified rhodamine B chemosensor, NRC) and preparation and use thereof. The naphthalimide-modified rhodamine derivative (naphthalimide modified rhodamine B chemosensor, NRC) is shown by a formula 1, and is prepared by the following steps : reacting N-butyl-4-bromo-1, 8-naphthalimide with 2, 4-dihydroxybenzaldehyde in excessive N, N-dimethylformamide (DMF) at 80 to 90 DEG C for 10 to 12 hours, and purifying the reaction product by a silica gel chromatographic column to obtain a yellow solid which is an intermediate compound for later use, wherein the molar ratio of the N-butyl-4-bromo-1, 8-naphthalimide to the 2, 4-dihydroxybenzaldehyde is 1 : 1 to 1 : 1.5; and performing the reflux reaction of the intermediate compound and the product of the hydrazinolysis of rhodamine B in absolute ethanol for 10 to 12 hours, filtering hot solution to obtain yellow powder, washing the yellow powder with boiling ethanol and diethyl ether in turn, and drying to obtain the naphthalimide-modified rhodamine derivative (naphthalimide modified rhodamine B chemosensor, NRC), wherein the molar ratio of the intermediate compound to the product of the hydrazinolysis of rhodamine B in the absolute ethanol is 1 : 1 to 1 : 1.5. In the invention, the probes are obtained by an effective synthesis method, the copper ion selectivity is high, and the detection of copper ions can be realized on the basis of optimization of experiment foundation.
; 于春伟; 张军
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