The invention relates to the technical field of detection of pathogenic bacteria of cultured sea cucumber skin disease syndromes, and particularly relates to a primer for rapidly detecting pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease. The invention is used for overcoming the defects of the technology for detecting main pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease syndromes of the existing sea cucumber, and providing a method for rapidly detecting pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease syndrome. The method comprises the following steps : centrifuging, cleaning, freezing and thawing and boiling a pure bacterial culture as a template to carry out LAMP (loop-mediated isothermal amplification); designing an LAMP primer aiming at a vibrio splendidus flgF gene; configuring and optimizing a reaction system; bathing for 45 minutes at the temperature of 65 DEG C, and judging the result by naked eyes or electrophoresis. By adopting the method, the LAMP technology is firstly applied to detection of pathogenic bacteria, namely vibrio splendidus of the sea cucumber skin disease syndromes. The method has the advantages of being short in time consumption, low in cost, simple, convenient and easy to use, suitable for popularization in a basic level, and the like.
; 秦松
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