The invention belongs to the biomedicine technical field, and concretely relates to Kunyuenin, a preparation method and an application. The Kunyuenin is cyclic polypeptide, the molecular weight is 1584.97Da and the isoelectric point is 8.96. The molecular weight of the Kunyuenin is small, the Kunyuenin has strong killing effect on staphylococcus aureus and Nocardia asteroids, and has low hemolytic activity and certain antioxidant activity.
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