The invention belongs to the technical field of biomedicine and particularly relates to a rana kunyuensis kunyuenin kunyuenin-3 as well as a preparation method and application of the rana kunyuensis kunyuenin kunyuenin-3. The kunyuenin-3 is the rana kunyuensis kunyuenin kunyuenin-3, is linear-chain polypeptide and contains 14 amino acid residues, the molecular weight is 1587.97Da, and the isoelectric point is 8.96. The kunyuenin-3 has high antioxidant activity, and has the beneficial characteristics that the molecular weight is small, the structure is simple, the hemolytic activity is low, the preparation method is simple, and the like.
; 于海宁; 秦松
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