The invention relates to the field of detecting environment pollutants by utilizing biological materials, and concretely relates to a method for rapidly detecting trace Cu. Trace Cu in a to-be detected solution is rapidly sensitively detected by utilizing the inhibition effect of Cu on a nucleophilic substitution reaction of L-cysteine and 1-chloro-2, 4-dinitrobenzene in boiling water bath. The method is simple and convenient in operation, rapid and visual by naked eyes, low in cost, wide in applicable samples and the like.
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