The invention relates to polymer sensitive membrane ion selective electrodes, in particular to a method for simultaneous detection of two substances.The method includes : inserting a polymer sensitive membrane ion selective electrode into a measuring cell of buffer solution containing two substrates, and detecting existing ions in the buffer solution in the measuring cell to obtain contrast potential signals of the existing ions different in electric property; adding functional magnetic beads for immobilized modification into the measuring cell, allowing enzyme reaction with the substrates at the room temperature to obtain indication ions different in electric property after enzyme reaction, and enabling the electrodes to capture potential signals of the indication ions different in electric property, namely obtaining the potential signals of the indication ions different in electric property after reaction with detected substances; indirectly and quantitatively obtaining concentrations of the detected substances according to the fact that the difference between the potential signals of the indication ions different in electric property after reaction with detected substances and the contrast potential signals of the existing ions different in electric property is caused by different charge indication ions generated after specific chemical reactions.By combination of a constant current technology and a constant potential technology, detection of the two substances is realized.
; 俞娜娜
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