The invention provides a thermo-sensitive western blot sensing microsphere based on a quantum dot and a preparation method of same and belongs to the technical field of material science and bio-separation engineering. The method includes the steps of : 1) with a functional protein molecule as a template molecule, combining the functional protein molecule with CdTe quantum dots, which are co-modified with glutathione-thioglycollic acid (GSH-TGA), through electrostatic charge effect by means of amino groups and carboxyl groups, which are rich in the functional protein molecule; 2) in a non-oxygenenvironment, initiating polymerization by means of potassium persulfate (KPS), wherein thermo-sensitive isopropyl acrylamide (NIPAM) is used as a functional monomer and N, N-methylene bisacrylamide (MBA) is used as a crosslinker, so that through the free radical polymerization, the thermo-sensitive western blot sensing microsphere based on the quantum dot is produced. Through mechanism of electrontransfer between the functional protein and the quantum dot, fluorescent sensing of the microspheres to the template protein is achieved, and the synthesized western blot microspheres has performances such as thermo-sensitive response, fluorescent detection, etc.
; 王晓艳; 陈令新
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