The invention relates to the field of pesticides, and particularly relates to a cadinane sesquiterpene compound derived from alga epiphytic fungi, a preparation method of the cadinane sesquiterpene compound and an application of the cadinane sesquiterpene compound in killing pests. The specific structural formula of the cadinane sesquiterpene compound is the formula (I). The preparation method comprises the following steps : inoculating Trichoderma virens Y13-3 in a fungal culture medium for fermentation culture, and separating and purifying the fermentation product to obtain the cadinane sesquiterpene compound represented by the formula (I). According to the cadinane sesquiterpene compound provided by the invention, the medium lethal concentration to artemia is 21 microgram/milliliter according to the insecticidal activity experiment.
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