The present invention belongs to the field of soil remediation technology, specifically relates to a preparation for the remediation of petroleum hydrocarbon pollutants and its application. The preparation is microorganisms fixed in the biochar material obtained by cracking; Among them, the single load of microorganisms in carbon materials was 3.2-6.5×1010cfu/g; The microorganisms are Acinetobacter HC8-3S and Dietssiella CN-3. The surfactant cleaning solution compounded by the present invention has high cleaning efficiency and small soil residue. The prepared biochar immobilized microbial materials have the advantages of high fixation rate, low production cost, promotion of soil microecological environment improvement, and no secondary pollution to the environment, and are used for the remediation of contaminated soil by petroleum and petroleum products.
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