The invention relates to the technical field of marine environment petroleum pollution control, in particular to a high-efficiency screening method of marine petroleum degrading bacteria, and preparation and application of a composite microbial inoculum. The composite bacterial agent contains a mixed strain of Pseudoxanthomonas (Pseudoxanthomonas sp.S1-2), Acinetobacter (Acinetobacter sp.HC8-3S) and Dietzia (Dietzia sp.CN-3). The marine oil degradation composite bacterial agent disclosed by the invention has a good degradation effect on oil in a neutral and slightly alkaline environment, can tolerate a high-salt environment, is particularly suitable for marine environment, and widens the application range and application prospect in marine oil pollution control.
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