The present invention belongs to the field of microbial product technology, and is especially one kind of composite microbial agent for improving aquiculture water and its preparation process. The microbial inoculum is the fermentation product of bacillus subtilis, rhodopseudomonas palustris, bacillus megaterium, lactobacillus acidophilus, streptococcus faecalis, bacillus circulans, candida utilis, nitrifying bacteria and denitrifying bacteria. The composite microbial agent of the present invention contains microbes capable of providing dissolved oxygen fast, microbes capable of decomposing ammonia nitrogen, hydrogen sulfide and nitrite in water, and bacterial strain capable of providing aquatic animal with nutritive matter required for production and development. Each strain has complementary advantages and synergistic effect; After being further applied to a water body, the water body can be rapidly propagated to form a dominant population, so that macromolecular organic matters, residual baits, excrement and the like in the aquaculture water body can be rapidly decomposed, substances such as ammonia nitrogen, phosphorus, nitrite, hydrogen sulfide and the like in the water body can be degraded, the water quality can be purified, the dissolved oxygen content of the water body can be improved, and pathogenic bacteria can be removed.
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