本发明涉及生物基因工程技术领域，是一种构建雨生红球藻叶绿体表达系统的方法。以雨生红球藻叶绿体基因组上SEQ ID NO : 1所示序列和SEQ ID NO : 2所示的序列为同源臂，以SEQ ID NO : 3所示的序列、SEQ ID NO : 4所示的序列为启动子，以SEQ ID NO : 5所示的序列、SEQ ID NO : 6所示的序列为终止子，以bar基因为筛选标记基因构建同源重组载体，并利用基因枪法将载体导入雨生红球藻细胞，经除草剂草丁膦筛选获得转基因藻株。采用本发明的雨生红球藻叶绿体稳定表达系统，可实现外源基因在叶绿体中稳定表达。
其他摘要
The invention relates to the technical field of biological genetic engineering, in particular to a haematococcus pluvialis chloroplast expression system and application thereof. A sequence as shown inSEQ ID NO : 1 and a sequence as shown in SEQ ID NO : 2 on a haematococcus pluvialis chloroplast genome are used as homologous arms, a sequence as shown in SEQ ID NO : 3 and sequence as shown in SEQ ID NO : 4are used as promoters, a sequence as shown in SEQ ID NO : 5 and a sequence as shown in SEQ ID NO : 6 are used as a terminator, a bar gene is used as a selective marker gene to build a homologous recombination carrier, a particle bombardment is used for guiding the carrier to haematococcus pluvialis cells, and transgenic algal plants are obtained through screening of herbicide glyphosate. By adoptingthe stable haematococcus pluvialis chloroplast expression system, stable expression of exogenous genes in a chloroplast can be realized.
; 王寅初
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