The invention belongs to the field of analytical chemistry and rapid detection, and particularly relates to a red/yellow/blue three-fluorescence emission sensor, a preparation method thereof and an application thereof in accurate visual detection of alkaline phosphatase. The sensor is composed of manganese dioxide nanometer sheet, o-phenylenediamine, red fluorescent quantum dot and buffer solution; Alternatively, the sensor consists of a manganese dioxide nanosheet, o-phenylenediamine, red fluorescent quantum dots and a buffer solution containing a substrate, wherein the substrate is L-ascorbic acid-phosphoric acid. The red-yellow-blue three-fluorescence emission sensor can be excited by the same wavelength, The fluorescence with three different wavelengths shows yellow fluorescence peak reduction, red fluorescence peak enhancement and blue fluorescence peak enhancement for alkaline phosphatase with gradually increased activity, The overall fluorescent color change is'yellow-orange-red-purple 'for visually detecting the content of the target object, and the content of the target object can also be measured by measuring the fluorescence intensities at three different emission wavelengths and taking the intensity change as a signal parameter.
; 杨倩; 陈令新
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