The invention discloses an aerobic denitrification strain and a microbial inoculum thereof. The aerobic denitrification strain and the microbial inoculum thereof are prepared by the following steps of : screening to obtain new strains of acinetobacter YTLJ-N-S 10 and bacillus YTLJ-N-B 38, respectively pre-culturing and propagating, carrying out vacuum freeze drying to obtain corresponding single bacterium powder, mixing the single bacterium powder, and then mixing the single bacterium powder with an aerobic denitrification bacterium extracellular polysaccharide crude product and auxiliary materials. The acinetobacter YTLJ-N-S 10 and the bacillus YTLJ-N-B 38 are preserved in the microbial strain preservation center of Guandong province, and the preservation numbers are GDMCC No : 61690 and GDMCC No : 61774 respectively. The acinetobacter YTLJ-N-S 10 and the bacillus YTLJ-N-B 38 are preserved in the microbial strain preservation center of Guandong province. The strain and the microbial inoculum thereof can enhance the heterotrophic nitrification and aerobic denitrification effects of a water body, simultaneously have the effect of inhibiting pathogenic bacteria, and can be used for purifying various sewage and enhancing the removal of nitrogen pollutants.
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