The present invention relates to a high-accuracy calcium ion detection method and its equipment. The device is an electrochemical device comprising a chemiluminescent flow injection system and an electrochemical external measurement device; The electrochemical external measuring device is connected with the electrode of the chemiluminescence flow injection system through a wire; The chemiluminescence flow injection system consists of a carrier liquid pool, a chemiluminescence coreactant pool, a peristaltic pump, a sample injection valve, a chemiluminescence measuring device and a waste liquid pool; The output pipelines of the liquid carrying pool and the chemiluminescence co-reactant pool are connected in parallel and then are connected with one end of the chemiluminescence measuring device through a main pipeline, the main pipeline is provided with a sampling valve, and the other end of the chemiluminescence measuring device is connected with the waste liquid pool. The device is combined with a flow injection system to control the diffusion of released gloss essence in the ion-carrier-free sensitive membrane under the action of an external constant potential, so as to realize the continuous detection of calcium ions. Said invention can implement high-sensitivity, high-accuracy and high-stability detection of calcium ion.
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