The present invention discloses a rotary microfluidic cloth/paper composite chip for detecting lead and mercury ions and its preparation method. The composite chip consists of bottom sensor layer, middle layer, top sampling layer, lead ion imprinting fluorescent sensing cloth and mercury ion imprinting fluorescent sensing cloth. The first three layers are made of filter paper printed with hydrophobic patterns and are stacked in sequence from bottom to top, the bottom sensor layer is provided with a detection area, the hydrophilic channel of the middle layer is matched with the detection area, the hydrophilic sampling area of the top sampling layer is integrally V-shaped and matched with the hydrophilic channel, and a detection hole is formed between two adjacent hydrophilic sampling areas; The latter two are respectively made of cloth pieces connected with mercury ion imprints or lead ion imprints, and both are adhered to the detection area of the bottom sensor layer and are arranged at intervals. The rotary micro-fluidic cloth/paper composite chip provided by the invention well combines the characteristics of a paper chip and cloth, is convenient to use, and can specifically and sensitively detect lead and mercury ions.
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